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The effect of Acellularized Wharton's Jelly-derived exosomes on myeloid differentiation of umbilical cord blood-derived CD34+ hematopoietic stem cells
Hematopoietic stem cell transplantation (HSCT) has been introduced as an effective treatment for hematological malignancies. Extracellular vesicles including exosomes and microvesicles, have recently attracted researcher's attention as the main factor in proliferation and differentiation of hem...
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| Published in: | Gene reports 2021-12, Vol.25, p.101298, Article 101298 |
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| cites | cdi_FETCH-LOGICAL-c306t-c464c95ce6a5cc5a19ce570adabfe6b76178924e37860493f838735d2d5088963 |
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| container_start_page | 101298 |
| container_title | Gene reports |
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| creator | Abbaszadeh, Hossein Ghorbani, Farzaneh Derakhshani, Mehdi Abbasi, Batoul Jalili, Zahra Talebi, Mehdi Yousefi, Mehdi Shamsasenjan, Karim Edalati, Mahdi Hakimi, Parvin Sanei, Maryam Yaghoubi, Reza Movassaghpour, Ali Akbar |
| description | Hematopoietic stem cell transplantation (HSCT) has been introduced as an effective treatment for hematological malignancies. Extracellular vesicles including exosomes and microvesicles, have recently attracted researcher's attention as the main factor in proliferation and differentiation of hematopoietic stem cells (HSCs) to accelerate the transplantation. Accordingly, this study aimed to evaluate the effect of exosomes derived from acellular Wharton’'s Jelly (WJ) on myeloid differentiation of umbilical cord blood (UCB)-derived CD34+ HSCs.
After isolation of CD34+ cells from human UCB via magnetic-activated cell sorting, these cells were cultured and incubated for three weeks. Then, UC-derived WJ was acellularized and used for exosome isolation. Finally, these exosomes were co-cultured with CD34+ cells and their differentiation to myeloid lineage was assessed by flow cytometry and Real-Time PCR.
The expression of CD33 was higher in the exosome group than that of the control group on day 0 (P |
| doi_str_mv | 10.1016/j.genrep.2021.101298 |
| format | article |
| fullrecord | <record><control><sourceid>elsevier_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1016_j_genrep_2021_101298</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S2452014421002831</els_id><sourcerecordid>S2452014421002831</sourcerecordid><originalsourceid>FETCH-LOGICAL-c306t-c464c95ce6a5cc5a19ce570adabfe6b76178924e37860493f838735d2d5088963</originalsourceid><addsrcrecordid>eNp9kE9LwzAYxoMoOHTfwENuHqQzadO0vQhj_mfgZeIxpMlbl5E2I-mG84P4eU2piCdP78sDz4-HH0IXlMwoofx6M3uHzsN2lpKUDlFalUdokrI8TQhl7PjPf4qmIWwIib2CVgWboK_VGjA0DageuwbPFVi7s9KbT9D4bS1977rLgJ9jfEg0eLOPOXy44FoI2HW4PYB1RmNtIsRD1xvZm5hH2K6tjTVKWqyc17i2zulfxuI2Y1d4Da3s3dYZ6I3CoYcWDwvCOTpppA0w_bln6PX-brV4TJYvD0-L-TJRGeF9ohhnqsoVcJkrlUtaKcgLIrWsG-B1wWlRVimDrCg5YVXWlFlZZLlOdU7KsuLZGWIjV3kXgodGbL1ppT8ISsSgV2zEqFcMesWoN9ZuxhrEbXsDXgRloFOgjY8mhXbmf8A3CxWHiw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>The effect of Acellularized Wharton's Jelly-derived exosomes on myeloid differentiation of umbilical cord blood-derived CD34+ hematopoietic stem cells</title><source>ScienceDirect Journals</source><creator>Abbaszadeh, Hossein ; Ghorbani, Farzaneh ; Derakhshani, Mehdi ; Abbasi, Batoul ; Jalili, Zahra ; Talebi, Mehdi ; Yousefi, Mehdi ; Shamsasenjan, Karim ; Edalati, Mahdi ; Hakimi, Parvin ; Sanei, Maryam ; Yaghoubi, Reza ; Movassaghpour, Ali Akbar</creator><creatorcontrib>Abbaszadeh, Hossein ; Ghorbani, Farzaneh ; Derakhshani, Mehdi ; Abbasi, Batoul ; Jalili, Zahra ; Talebi, Mehdi ; Yousefi, Mehdi ; Shamsasenjan, Karim ; Edalati, Mahdi ; Hakimi, Parvin ; Sanei, Maryam ; Yaghoubi, Reza ; Movassaghpour, Ali Akbar</creatorcontrib><description>Hematopoietic stem cell transplantation (HSCT) has been introduced as an effective treatment for hematological malignancies. Extracellular vesicles including exosomes and microvesicles, have recently attracted researcher's attention as the main factor in proliferation and differentiation of hematopoietic stem cells (HSCs) to accelerate the transplantation. Accordingly, this study aimed to evaluate the effect of exosomes derived from acellular Wharton’'s Jelly (WJ) on myeloid differentiation of umbilical cord blood (UCB)-derived CD34+ HSCs.
After isolation of CD34+ cells from human UCB via magnetic-activated cell sorting, these cells were cultured and incubated for three weeks. Then, UC-derived WJ was acellularized and used for exosome isolation. Finally, these exosomes were co-cultured with CD34+ cells and their differentiation to myeloid lineage was assessed by flow cytometry and Real-Time PCR.
The expression of CD33 was higher in the exosome group than that of the control group on day 0 (P < 0.001), however, the change in expression of CD33, in the presence of exosome was not significant in comparison with the control group on day 3 (P ≥ 0.05). Also, the expression of myeloid-specific gene (RUNX1) and was reduced in the exosome group after three days (P < 0.05) and the expression of another myeloid-specific gene(C/EBP–α) had no significant difference with the control group (P ≥ 0.05).
Overall, these observations indicated that AWJ-exosomes may have no potential impact on myeloid differentiation of CD34+ HSCs derived from UCB.
[Display omitted]
•Decrease of CD33 between the days 0 to 3 in both control and exosome groups.•Reduction of RUNX1 and C/EBP–α in the presence of AWJ-exosomes in comparison with the control group.•AWJ-exosomes have no potential effects on the myeloid differentiation capacity of UCB-derived CD34+ cells in vitro due to the reduction of the expression of CD33 marker.</description><identifier>ISSN: 2452-0144</identifier><identifier>EISSN: 2452-0144</identifier><identifier>DOI: 10.1016/j.genrep.2021.101298</identifier><language>eng</language><publisher>Elsevier Inc</publisher><subject>Exosomes ; Extracellular vesicles ; Hematopoietic stem cells ; Myeloid ; Wharton's Jelly</subject><ispartof>Gene reports, 2021-12, Vol.25, p.101298, Article 101298</ispartof><rights>2021</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c306t-c464c95ce6a5cc5a19ce570adabfe6b76178924e37860493f838735d2d5088963</citedby><cites>FETCH-LOGICAL-c306t-c464c95ce6a5cc5a19ce570adabfe6b76178924e37860493f838735d2d5088963</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S2452014421002831$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27901,27902,45756</link.rule.ids></links><search><creatorcontrib>Abbaszadeh, Hossein</creatorcontrib><creatorcontrib>Ghorbani, Farzaneh</creatorcontrib><creatorcontrib>Derakhshani, Mehdi</creatorcontrib><creatorcontrib>Abbasi, Batoul</creatorcontrib><creatorcontrib>Jalili, Zahra</creatorcontrib><creatorcontrib>Talebi, Mehdi</creatorcontrib><creatorcontrib>Yousefi, Mehdi</creatorcontrib><creatorcontrib>Shamsasenjan, Karim</creatorcontrib><creatorcontrib>Edalati, Mahdi</creatorcontrib><creatorcontrib>Hakimi, Parvin</creatorcontrib><creatorcontrib>Sanei, Maryam</creatorcontrib><creatorcontrib>Yaghoubi, Reza</creatorcontrib><creatorcontrib>Movassaghpour, Ali Akbar</creatorcontrib><title>The effect of Acellularized Wharton's Jelly-derived exosomes on myeloid differentiation of umbilical cord blood-derived CD34+ hematopoietic stem cells</title><title>Gene reports</title><description>Hematopoietic stem cell transplantation (HSCT) has been introduced as an effective treatment for hematological malignancies. Extracellular vesicles including exosomes and microvesicles, have recently attracted researcher's attention as the main factor in proliferation and differentiation of hematopoietic stem cells (HSCs) to accelerate the transplantation. Accordingly, this study aimed to evaluate the effect of exosomes derived from acellular Wharton’'s Jelly (WJ) on myeloid differentiation of umbilical cord blood (UCB)-derived CD34+ HSCs.
After isolation of CD34+ cells from human UCB via magnetic-activated cell sorting, these cells were cultured and incubated for three weeks. Then, UC-derived WJ was acellularized and used for exosome isolation. Finally, these exosomes were co-cultured with CD34+ cells and their differentiation to myeloid lineage was assessed by flow cytometry and Real-Time PCR.
The expression of CD33 was higher in the exosome group than that of the control group on day 0 (P < 0.001), however, the change in expression of CD33, in the presence of exosome was not significant in comparison with the control group on day 3 (P ≥ 0.05). Also, the expression of myeloid-specific gene (RUNX1) and was reduced in the exosome group after three days (P < 0.05) and the expression of another myeloid-specific gene(C/EBP–α) had no significant difference with the control group (P ≥ 0.05).
Overall, these observations indicated that AWJ-exosomes may have no potential impact on myeloid differentiation of CD34+ HSCs derived from UCB.
[Display omitted]
•Decrease of CD33 between the days 0 to 3 in both control and exosome groups.•Reduction of RUNX1 and C/EBP–α in the presence of AWJ-exosomes in comparison with the control group.•AWJ-exosomes have no potential effects on the myeloid differentiation capacity of UCB-derived CD34+ cells in vitro due to the reduction of the expression of CD33 marker.</description><subject>Exosomes</subject><subject>Extracellular vesicles</subject><subject>Hematopoietic stem cells</subject><subject>Myeloid</subject><subject>Wharton's Jelly</subject><issn>2452-0144</issn><issn>2452-0144</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kE9LwzAYxoMoOHTfwENuHqQzadO0vQhj_mfgZeIxpMlbl5E2I-mG84P4eU2piCdP78sDz4-HH0IXlMwoofx6M3uHzsN2lpKUDlFalUdokrI8TQhl7PjPf4qmIWwIib2CVgWboK_VGjA0DageuwbPFVi7s9KbT9D4bS1977rLgJ9jfEg0eLOPOXy44FoI2HW4PYB1RmNtIsRD1xvZm5hH2K6tjTVKWqyc17i2zulfxuI2Y1d4Da3s3dYZ6I3CoYcWDwvCOTpppA0w_bln6PX-brV4TJYvD0-L-TJRGeF9ohhnqsoVcJkrlUtaKcgLIrWsG-B1wWlRVimDrCg5YVXWlFlZZLlOdU7KsuLZGWIjV3kXgodGbL1ppT8ISsSgV2zEqFcMesWoN9ZuxhrEbXsDXgRloFOgjY8mhXbmf8A3CxWHiw</recordid><startdate>202112</startdate><enddate>202112</enddate><creator>Abbaszadeh, Hossein</creator><creator>Ghorbani, Farzaneh</creator><creator>Derakhshani, Mehdi</creator><creator>Abbasi, Batoul</creator><creator>Jalili, Zahra</creator><creator>Talebi, Mehdi</creator><creator>Yousefi, Mehdi</creator><creator>Shamsasenjan, Karim</creator><creator>Edalati, Mahdi</creator><creator>Hakimi, Parvin</creator><creator>Sanei, Maryam</creator><creator>Yaghoubi, Reza</creator><creator>Movassaghpour, Ali Akbar</creator><general>Elsevier Inc</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>202112</creationdate><title>The effect of Acellularized Wharton's Jelly-derived exosomes on myeloid differentiation of umbilical cord blood-derived CD34+ hematopoietic stem cells</title><author>Abbaszadeh, Hossein ; Ghorbani, Farzaneh ; Derakhshani, Mehdi ; Abbasi, Batoul ; Jalili, Zahra ; Talebi, Mehdi ; Yousefi, Mehdi ; Shamsasenjan, Karim ; Edalati, Mahdi ; Hakimi, Parvin ; Sanei, Maryam ; Yaghoubi, Reza ; Movassaghpour, Ali Akbar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c306t-c464c95ce6a5cc5a19ce570adabfe6b76178924e37860493f838735d2d5088963</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Exosomes</topic><topic>Extracellular vesicles</topic><topic>Hematopoietic stem cells</topic><topic>Myeloid</topic><topic>Wharton's Jelly</topic><toplevel>online_resources</toplevel><creatorcontrib>Abbaszadeh, Hossein</creatorcontrib><creatorcontrib>Ghorbani, Farzaneh</creatorcontrib><creatorcontrib>Derakhshani, Mehdi</creatorcontrib><creatorcontrib>Abbasi, Batoul</creatorcontrib><creatorcontrib>Jalili, Zahra</creatorcontrib><creatorcontrib>Talebi, Mehdi</creatorcontrib><creatorcontrib>Yousefi, Mehdi</creatorcontrib><creatorcontrib>Shamsasenjan, Karim</creatorcontrib><creatorcontrib>Edalati, Mahdi</creatorcontrib><creatorcontrib>Hakimi, Parvin</creatorcontrib><creatorcontrib>Sanei, Maryam</creatorcontrib><creatorcontrib>Yaghoubi, Reza</creatorcontrib><creatorcontrib>Movassaghpour, Ali Akbar</creatorcontrib><collection>CrossRef</collection><jtitle>Gene reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abbaszadeh, Hossein</au><au>Ghorbani, Farzaneh</au><au>Derakhshani, Mehdi</au><au>Abbasi, Batoul</au><au>Jalili, Zahra</au><au>Talebi, Mehdi</au><au>Yousefi, Mehdi</au><au>Shamsasenjan, Karim</au><au>Edalati, Mahdi</au><au>Hakimi, Parvin</au><au>Sanei, Maryam</au><au>Yaghoubi, Reza</au><au>Movassaghpour, Ali Akbar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effect of Acellularized Wharton's Jelly-derived exosomes on myeloid differentiation of umbilical cord blood-derived CD34+ hematopoietic stem cells</atitle><jtitle>Gene reports</jtitle><date>2021-12</date><risdate>2021</risdate><volume>25</volume><spage>101298</spage><pages>101298-</pages><artnum>101298</artnum><issn>2452-0144</issn><eissn>2452-0144</eissn><abstract>Hematopoietic stem cell transplantation (HSCT) has been introduced as an effective treatment for hematological malignancies. Extracellular vesicles including exosomes and microvesicles, have recently attracted researcher's attention as the main factor in proliferation and differentiation of hematopoietic stem cells (HSCs) to accelerate the transplantation. Accordingly, this study aimed to evaluate the effect of exosomes derived from acellular Wharton’'s Jelly (WJ) on myeloid differentiation of umbilical cord blood (UCB)-derived CD34+ HSCs.
After isolation of CD34+ cells from human UCB via magnetic-activated cell sorting, these cells were cultured and incubated for three weeks. Then, UC-derived WJ was acellularized and used for exosome isolation. Finally, these exosomes were co-cultured with CD34+ cells and their differentiation to myeloid lineage was assessed by flow cytometry and Real-Time PCR.
The expression of CD33 was higher in the exosome group than that of the control group on day 0 (P < 0.001), however, the change in expression of CD33, in the presence of exosome was not significant in comparison with the control group on day 3 (P ≥ 0.05). Also, the expression of myeloid-specific gene (RUNX1) and was reduced in the exosome group after three days (P < 0.05) and the expression of another myeloid-specific gene(C/EBP–α) had no significant difference with the control group (P ≥ 0.05).
Overall, these observations indicated that AWJ-exosomes may have no potential impact on myeloid differentiation of CD34+ HSCs derived from UCB.
[Display omitted]
•Decrease of CD33 between the days 0 to 3 in both control and exosome groups.•Reduction of RUNX1 and C/EBP–α in the presence of AWJ-exosomes in comparison with the control group.•AWJ-exosomes have no potential effects on the myeloid differentiation capacity of UCB-derived CD34+ cells in vitro due to the reduction of the expression of CD33 marker.</abstract><pub>Elsevier Inc</pub><doi>10.1016/j.genrep.2021.101298</doi></addata></record> |
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| source | ScienceDirect Journals |
| subjects | Exosomes Extracellular vesicles Hematopoietic stem cells Myeloid Wharton's Jelly |
| title | The effect of Acellularized Wharton's Jelly-derived exosomes on myeloid differentiation of umbilical cord blood-derived CD34+ hematopoietic stem cells |
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