Association between GPX1 and IL-6 promoter methylation and type 2 diabetes

Type 2 diabetes (T2D) is a multifactorial illness, and the development of this disease is the consequence of combination of the type of lifestyle and various genetic factors. Epigenetic changes occur due to interference between environmental and lifestyle factors. Epigenetic can affect the function...

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Bibliographic Details
Published in:Gene reports 2022-03, Vol.26, p.101530, Article 101530
Main Authors: Ravari, Mehrnaz sadat, Moradi, Zahra, Farrokhi, Effat, Chaleshtori, Morteza Hashemzadeh, Jami, Mohammad-saeid, Zarandi, Mehdi Borhani
Format: Article
Language:English
Subjects:
Diabetes type 2
DNA methylation
Glutathione Peroxidase 1
Interleukin 6
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Summary:Type 2 diabetes (T2D) is a multifactorial illness, and the development of this disease is the consequence of combination of the type of lifestyle and various genetic factors. Epigenetic changes occur due to interference between environmental and lifestyle factors. Epigenetic can affect the function of the genes involved in the pathway of glucose metabolism. In the present study, the promoter methylation levels of two candidate genes (IL-6 and GPX1) in patients with T2D and non-diabetic individuals were analyzed. Total 62 patients with T2D and 30 age- and gender-had similar healthy controls were included. Lipid profiles were enzymatically measured and DNA extraction, bisulfite conversion, nested PCR and sequencing were performed to investigate the DNA methylation in IL-6 and GPX1. No differences were observed for the IL-6 and GPX1 promoter methylation between T2D and non-diabetic subjects. However, our results revealed that the level of methylation of GPX1 promoter was significantly higher in females, compared with males (p 
ISSN:2452-0144
2452-0144
DOI:10.1016/j.genrep.2022.101530