P060 Antibody-mediated and cellular rejection: How can they seem so similar?

Aim Antibody-mediated rejection (ABMR) involves a cellular component where NK cell CD16 activation plays a pivotal role. In T-cell mediated rejection (TCMR) CD8+ T cells; the most closely related cell to NK cells, are most prevalent. We used global gene expression analyses to determine the similarit...

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Bibliographic Details
Published in:Human immunology 2016-09, Vol.77, p.82-82
Main Authors: Parkes, Michael, Halloran, Phil F, Hidalgo, Luis G
Format: Article
Language:English
Subjects:
Allergy and Immunology
Online Access:Get full text
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Summary:Aim Antibody-mediated rejection (ABMR) involves a cellular component where NK cell CD16 activation plays a pivotal role. In T-cell mediated rejection (TCMR) CD8+ T cells; the most closely related cell to NK cells, are most prevalent. We used global gene expression analyses to determine the similarities in gene expression changes following CD16 and CD3 stimulation of NK cells and CD8+ T cells, respectively and examined their expression in kidney transplant biopsies. Methods Cells were purified from PBMCs by immunomagnetic separation and stimulated in vitro on plates coated with crosslinking antibodies or uncoated plates. NK (N = 3) and CD8+ T cells (N = 3) were cultured for 4 h in the presence of IL2 prior to harvest. RNA was extracted and analyzed on Affymetrix gene expression arrays and supernatants underwent multiplex analysis for soluble mediators. Results We identified 276 transcripts increased (>2X, FDR
ISSN:0198-8859
1879-1166
DOI:10.1016/j.humimm.2016.07.125