Bioscorodite crystallization using Acidianus brierleyi: Effects caused by Cu(II) present in As(III)-bearing copper refinery wastewaters

• Published in: Hydrometallurgy 2017-03, Vol.168, p.121-126
• Main Authors: Okibe, Naoko, Morishita, Shiori, Tanaka, Masahito, Sasaki, Keiko, Hirajima, Tsuyoshi, Hatano, Kazuhiro, Ohata, Atsuko
• Format: Article
• Language: English
• Subjects: Acidianus brierleyi; Acidophile; As(III)-oxidation; Fe(II)-oxidation; Scorodite
• ISSN: 0304-386X; 1879-1158
• DOI: 10.1016/j.hydromet.2016.07.003

This study investigated the effect caused by Cu(II) in synthetic As(III)-bearing copper refinery wastewaters, on microbial scorodite (FeAsO4·2H2O) formation using the thermo-acidophilic Fe(II)- and As(III)-oxidizing archaeon, Acidianus brierleyi. Microbial Fe(II) oxidation and cell growth became only marginal in the presence of 8–16mM Cu(II), with its As(III) oxidation ability being severely inhibited. Consequently, scorodite formation was disabled by Cu(II) addition. However, feeding scorodite seed crystals readily alleviated Fe(II)- and As(III)-oxidation ability of Ac. brierleyi at 8mM Cu(II), forming crystalline scorodite within 24days in shake flasks. Zeta potential analysis indicated cell attachment to the scorodite seed crystal surface, implying its role in providing the immediate support for microbial colonization and enabling more robust microbial reactions. Most of Cu(II) was neither adsorbed nor co-precipitated and remained in the solution phase during scorodite crystallization, with or without the presence of seed crystals. Addition of seed crystals at 0.015, 0.03, 0.075 and 0.15% resulted in As immobilization of 96, 97, 97 and 98%, respectively, by day 24. This study demonstrated that despite of its inhibitory effect on Ac. brierleyi cells, scorodite can still be crystallized in the presence of Cu(II) by feeding scorodite seeds from synthetic copper refinery As(III)-bearing wastewaters. •Cu(II) is inhibitory to As(III) and Fe(II) oxidation ability in Ac. brierleyi.•Feeding scorodite seeds crystals effectively alleviate the above inhibitory effect.•Scorodite seed crystals provide support for microbial colonization and enable robust microbial reactions.•The yield and crystallinity of bioscorodite were comparable regardless of the presence of Cu(II).