Use of DEAE cellulose adsorbed and crosslinked white radish ( Raphanus sativus) peroxidase for the removal of α-naphthol in batch and continuous process

• Published in: International biodeterioration & biodegradation 2010, Vol.64 (1), p.27-31
• Main Authors: Ashraf, Humaira, Husain, Qayyum
• Format: Article
• Language: English
• Subjects: DEAE cellulose; Immobilization; Peroxidase; Polyethylene glycol; Wastewater
• ISSN: 0964-8305; 1879-0208
• DOI: 10.1016/j.ibiod.2009.10.003

DEAE cellulose bound white radish peroxidase was employed for the treatment of wastewater contaminated with α-naphthol in batch as well as in continuous reactor. Immobilized enzyme could effectively remove about 79%, 87%, 65% α-naphthol in stirred batch process at 30 °C, 40 °C and 50 °C after 5 h treatment, respectively. However, its soluble counterpart under similar experimental conditions could remove 73%, 75%, 53% α-naphthol. FTIR spectra of the obtained product showed the occurrence of polymerization reaction involving C–O–C bond, Immobilized WRP could remove 64% α-naphthol in presence of 3.0 mM CdCl 2; however, its soluble counterpart oxidized only 44% of the contaminant. Immobilized preparation after its 6th repeated use was able to oxidize 58% α-naphthol. Immobilized enzyme present in the column was used for the continuous removal of α-naphthol and the column retained 57% α-naphthol removal efficiency after one month operation. The absorption spectra of the treated and untreated α-naphthol containing wastewater exhibited a marked difference in the absorption at various wavelengths. Hence, the designed column reactor could be used successfully at a large scale for treatment of water contaminated with various aromatic compounds.