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Rational design of mimetic peptides based on the interaction between Inga laurina inhibitor and trypsins for Spodoptera cosmioides pest control

The interaction of Inga laurina Kunitz inhibitor with insect trypsins is an example of protein-protein interaction with potential application for the pest control. However, the crop field application of proteins as inhibitors is limited due to high production cost, the large molecular size and low e...

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Bibliographic Details
Published in:Insect biochemistry and molecular biology 2020-07, Vol.122, p.103390, Article 103390
Main Authors: Meriño-Cabrera, Yaremis, Severiche Castro, José Gregório, Rios Diez, Juan Diego, Rodrigues Macedo, Maria Ligia, de Oliveira Mendes, Tiago Antônio, Goreti de Almeida Oliveira, Maria
Format: Article
Language:English
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Summary:The interaction of Inga laurina Kunitz inhibitor with insect trypsins is an example of protein-protein interaction with potential application for the pest control. However, the crop field application of proteins as inhibitors is limited due to high production cost, the large molecular size and low environmental stability. The use of mimetic peptides that have molecular features associated with the protein inhibitor can result in a product with lower cost and higher efficiency for the agricultural application. Here, we designed mimetic peptides deriving from globular domains of ILTI that are predicted to interact with trypsin enzymes of Lepidoptera pest. Two linear peptides were identified and synthetized from the interface of interaction between trypsin-ILTI complexes. These peptides were derived due to its high-energy contribution for the biding affinity between the enzyme-protein inhibitor. The peptides showed structural stability, propensity to adopt the bound conformation also without the context of the protein, inhibitory activity of digestive trypsins and toxic effects on the S. cosmioides, indicating that they can be used as potential inhibitor for pest control. [Display omitted] •Two peptides based on the structure of Inga laurina inhibitor with potential to inhibit Lepidoptera trypsins were designed.•The peptides showed high stability and conservation of inhibitory activity of gut trypsins.•The peptides reduced of the survival of Spodoptera cosmioides indicating potential application for plant pest control.
ISSN:0965-1748
1879-0240
DOI:10.1016/j.ibmb.2020.103390