Dinuclear copper(II) complexes bridged with acetate and nitrate anions: Study of protein binding and in-vitro anticancer activity

• Published in: Inorganica Chimica Acta 2024-01, Vol.559, p.121799, Article 121799
• Main Authors: Shaikh, Sabiha A., Bhat, Satish S., Revankar, Vidyanand K., Naveen, S., Mahesha, Lokanath, N.K., Kumbar, Vijay
• Format: Article
• Language: English
• Subjects: 1,8-naphthalimide; Anticancer; Copper; Dinuclear; MTT assay
• ISSN: 0020-1693; 1873-3255
• DOI: 10.1016/j.ica.2023.121799

[Display omitted] •Dinuclear copper complexes containing N-(3-pyridyl)-1,8-naphthalimide ligand has been synthesized.•Complexes have distorted square planar geometry around copper center.•Four acetate ions and two nitrate ions bridges the two copper center in complex 1 and complex 2 respectively.•BSA binding of complexes follow dynamic quenching mechanism.•Anticancer activity of the complexes has been studied using an MTT assay. Two dinuclear copper(Ⅱ) complexes [Cu(L)(μ-AcO)2]2·H2O (1) and [Cu(L)(bpy)(μ-NO3)]2. (ClO4)2 (2) were synthesized with the ligand L = N-(3-pyridyl)-1,8-naphthalimide (where, OAc = Acetate, bpy = 2,2′-bipyridine). The complexes were characterized with FT-IR, UV–vis, fluorescence, EPR, and single crystal X-ray diffraction technique. Complex 1 contains two copper(Ⅱ) units bridged by four acetate ions via oxygen atoms having distorted square pyramidal geometry around both the copper(Ⅱ) centers. Complex 2 consists of two copper(Ⅱ) centers with two bridging nitrates via oxygen atoms in a distorted square pyramidal environment. The complexes 1 and 2 efficiently bind to BSA (bovine serum albumin) showing a dynamic quenching mechanism. The in-vitro anticancer activity have been investigated against HeLa cell lines using MTT assay. Complex 1 is two times more active than cisplatin under the same experimental conditions, whereas complex 2 is twelve times more active.