Rapid identification of the origin of the matrix protein gene in vaccine candidate reassortants using real-time PCR and fluorescence-based melting curve analysis

A simple and rapid method using real-time PCR and melting temperature analysis is described for the detection of the origin of the matrix gene in reassortant influenza A viruses. This procedure relies on differences between the matrix gene PCR product melting temperature of recent human A(H1N1) and...

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Bibliographic Details
Published in:International Congress series 2004-06, Vol.1263, p.581-584
Main Authors: Komadina, Naomi, Vasudevan, Sumitha, Johnstone, David, Barr, Ian, Hampson, Alan
Format: Article
Language:English
Subjects:
Influenza
Matrix gene
Melting temperature
Real-time PCR
Reassortants
Vaccine
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Description
Summary:A simple and rapid method using real-time PCR and melting temperature analysis is described for the detection of the origin of the matrix gene in reassortant influenza A viruses. This procedure relies on differences between the matrix gene PCR product melting temperature of recent human A(H1N1) and A(H3N2) strains and the laboratory donor strain PR-8 allowing the origin of the matrix gene to be identified. This novel method offers advantages over previous methods such as hybridisation and RFLP in facilitating rapid selection of candidate vaccine strains.
ISSN:0531-5131
1873-6157
DOI:10.1016/j.ics.2004.02.155