Insulin-like growth factors in rat leiomyoma and leiomyosarcoma

The molecular physiologic factors responsible for leiomyoma tumor initiation and growth are poorly understood. Gene expression of insulinlike growth factors (IGFs) is increased in leiomyoma and leiomyosarcoma. We examined the functional properties of the members of the IGF family in cell lines deriv...

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Bibliographic Details
Published in:International Congress series 2004-09, Vol.1271, p.287-290
Main Authors: Loret de Mola, J. Ricardo, Yi, Lijuan, Tan, Huiqing, Garza, Arturo, Giardina, Karen, Chernicky, Cheryl L.
Format: Article
Language:English
Subjects:
Eker rat uterine cell lines
Invasion
Transformation
Type I insulinlike growth factor receptor
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Summary:The molecular physiologic factors responsible for leiomyoma tumor initiation and growth are poorly understood. Gene expression of insulinlike growth factors (IGFs) is increased in leiomyoma and leiomyosarcoma. We examined the functional properties of the members of the IGF family in cell lines derived from uterine tumors in Eker rats (ELT3, ELT5A, ELT5B) for neoplastic characteristics using assays for cellular transformation and invasion. All cell lines tested expressed IGF-I and the type I IGF receptor (IGF-IR). IGF-II mRNA was detectable only in the ELT 5A cells. IGF-IR protein staining intensity was ELT3>ELT5A>ELT5B. Colony formation in soft agar was similar in ELT3 and ELT5B cells, whereas ELT5A cells did not form colonies. ELT3 cells displayed the most invasive phenotype followed by ELT5B. ELT5A cells were minimally invasive in Matrigel. These results show that there is a possible correlation between IGFs and neoplastic growth and cellular transformation in rat uterine cells.
ISSN:0531-5131
1873-6157
DOI:10.1016/j.ics.2004.07.004