New sensitive methods for fraud detection in buffalo dairy products

The rising consumption of buffalo milk derivatives in recent years has highlighted the search for buffaloes in Brazilian livestock farming. These products have greater added value compared with common dairy milk products. Consequently, addition of variable amounts of cows' milk during manufactu...

Full description

Saved in:
Bibliographic Details
Published in:International dairy journal 2021-06, Vol.117, p.105013, Article 105013
Main Authors: Azevedo, Bianca Tainá, Vercesi Filho, Anibal Eugênio, Gutmanis, Gunta, Verissimo, Cecília José, Katiki, Luciana Morita, Okino, Cintia Hiromi, Cristina de Sena Oliveira, Márcia, Giglioti, Rodrigo
Format: Article
Language:English
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The rising consumption of buffalo milk derivatives in recent years has highlighted the search for buffaloes in Brazilian livestock farming. These products have greater added value compared with common dairy milk products. Consequently, addition of variable amounts of cows' milk during manufacture of buffalo dairy products may occur, which constitutes fraud by product adulteration. Thus, the present study developed and standardised a DNA extraction protocol for application on different dairy derivatives and two methods based on real-time PCR for fraud identification: high-resolution melting (HRM) and rhAmp® SNP system. The extraction method allowed to extraction of DNA from eleven different dairy products. The sensitivity of the rhAmp method (1%) was five times higher than HRM assay (5%) and may be considered a better choice for identification of product adulteration when high sensitivity levels are required.
ISSN:0958-6946
1879-0143
DOI:10.1016/j.idairyj.2021.105013