Promising biocatalysts for producing lactose-free food matrices: β-galactosidase immobilized on cellulose-rich agro-industrial waste supports

This study aimed to develop, characterize, and apply wheat husk and rice husk-based supports for immobilizing β-galactosidase (β-gal) tagged with a Cellulose Binding Domain (β-gal-CBD) in column reactors. Biocatalysts were applied in skim milk hydrolysis in two column reactor configurations: balls c...

Full description

Saved in:
Bibliographic Details
Published in:Innovative food science & emerging technologies 2024-10, Vol.97, p.103803, Article 103803
Main Authors: Gennari, Adriano, Leonhardt, Fernanda, Tupuna-Yerovi, Diego Santiago, Benvenutti, Edilson Valmir, Nicolodi, Sabrina, Renard, Gaby, Chies, Jocelei Maria, Volpato, Giandra, Volken de Souza, Claucia Fernanda
Format: Article
Language:English
Subjects:
Alginate
Core-shell
Rice husk
Spheres
Wheat husk
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This study aimed to develop, characterize, and apply wheat husk and rice husk-based supports for immobilizing β-galactosidase (β-gal) tagged with a Cellulose Binding Domain (β-gal-CBD) in column reactors. Biocatalysts were applied in skim milk hydrolysis in two column reactor configurations: balls column reactor (BCR) and toothed column reactor (TCR). Spheres containing 15 % rice and wheat husk powders showed 10× and 25× increased hardness, respectively, compared to controls. Wheat husk-added core-shell capsules had the highest structural resistance. Immobilization of β-gal-CBD onto supports (3 U/sphere or 3 U/capsule) was achieved in 15 min, with yield and efficiency values of up to 90 %. After 48 h of continuous reaction in column reactors, the developed biocatalysts maintained over 55 % of their relative hydrolysis values, indicating significant stability. Utilizing these residues for enzyme immobilization supports introduces a novel application, contributing to waste reduction and adding value to agro-industrial residues while enhancing enzymatic biocatalysis. •Wheat and rice husk supports for oriented β-galactosidase immobilization;•Enhanced structural resistance of spheres containing rice and wheat husk powders;•Rapid enzyme immobilization with high yield and efficiency values of up to 90 %;•Biocatalysts maintain stability over 55 % after 48-h of lactose hydrolysis reaction;•Utilization of residues allows for enhanced biocatalysts and waste reduction.
ISSN:1466-8564
DOI:10.1016/j.ifset.2024.103803