Electrospun gelatin nanofibers: A facile cross-linking approach using oxidized sucrose

Gelatin nanofibers were fabricated via electrospinning with minimal toxicity from solvents and cross-linking agents. Electrospinning was carried out using a solvent system based on water and acetic acid (8:2, v/v). Acetic acid concentration was kept as minimum as possible to reduce the toxic effects...

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Bibliographic Details
Published in:International journal of biological macromolecules 2015-02, Vol.73, p.270-278
Main Authors: Jalaja, K., James, Nirmala R.
Format: Article
Language:English
Subjects:
Biocompatible Materials - chemistry
Cell Line
Cell Survival
Gelatin
Gelatin - chemistry
Humans
Mechanical Phenomena
Nanofibers
Nanofibers - chemistry
Nanofibers - ultrastructure
Oxidation-Reduction
Oxidized sucrose
Spectroscopy, Fourier Transform Infrared
Sucrose - chemistry
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Summary:Gelatin nanofibers were fabricated via electrospinning with minimal toxicity from solvents and cross-linking agents. Electrospinning was carried out using a solvent system based on water and acetic acid (8:2, v/v). Acetic acid concentration was kept as minimum as possible to reduce the toxic effects. Electrospun gelatin nanofibers were cross-linked with oxidized sucrose. Sucrose was oxidized by periodate oxidation to introduce aldehyde functionality. Cross-linking with oxidized sucrose could be achieved without compromising the nanofibrous architecture. Cross-linked gelatin nanofibers maintained the fibrous morphology even after keeping in contact with aqueous medium. The morphology of the cross-linked nanofibrous mats was examined by scanning electron microscopy (SEM). Oxidized sucrose cross-linked gelatin nanofibers exhibited improved thermal and mechanical properties. The nanofibrous mats were evaluated for cytotoxicity and cell viability using L-929 fibroblast cells. The results confirmed that oxidized sucrose cross-linked gelatin nanofibers were non-cytotoxic towards L-929 cells with good cell viability.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2014.11.018