In vitro and in vivo evaluation of anti-arthritic, antioxidant efficacy of fucoidan from Undaria pinnatifida (Harvey) Suringar

• Published in: International journal of biological macromolecules 2017-04, Vol.97, p.468-480
• Main Authors: Phull, Abdul-Rehman, Majid, Muhammad, Haq, Ihsan-ul, Khan, Muhammad Rashid, Kim, Song Ja
• Format: Article
• Language: English
• Subjects: Animals; Anti-arthritic; Antioxidant; Antioxidants - pharmacology; Antioxidants - therapeutic use; Antioxidants - toxicity; Arthritis, Rheumatoid - chemically induced; Arthritis, Rheumatoid - drug therapy; Arthritis, Rheumatoid - metabolism; Arthritis, Rheumatoid - pathology; Chondrocytes - drug effects; Chondrocytes - pathology; Cyclooxygenase-2; Edema - chemically induced; Edema - drug therapy; Freund's Adjuvant - pharmacology; Fucoidan; Hematologic Tests; Joints - drug effects; Joints - metabolism; Joints - pathology; Male; Oxidative Stress - drug effects; Polysaccharides - pharmacology; Polysaccharides - therapeutic use; Polysaccharides - toxicity; Rabbits; Rats; Rats, Sprague-Dawley; Undaria - chemistry
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2017.01.051

•Effect of fucoidan on Freund’s complete adjuvant-induced arthritis is studied.•Fucoidan improved the arthritis-induced physical, biochemical and hematological changes.•Fucoidan attenuated inflammatory responses in rats.•Fucoidan down regulated COX-2 in rabbit articular chondrocytes.•Fucoidan exhibited significant antioxidant activity. Seaweed and their constituents have been traditionally employed for the management of various human pathologic conditions such as edema, urinary disorders and inflammatory anomalies. The current study was performed to investigate the antioxidant and anti-arthritic effects of fucoidan from Undaria pinnatifida. A noteworthy in vitro antioxidant potential at 500μg/ml in 2, 2-diphenyl-1-picrylhydrazyl scavenging assay (80% inhibition), nitrogen oxide inhibition assay (71.83%), hydroxyl scavenging assay (71.92%), iron chelating assay (73.55%) and a substantial ascorbic acid equivalent reducing power (399.35μg/mg ascorbic acid equivalent) and total antioxidant capacity (402.29μg/mg AAE) suggested fucoidan a good antioxidant agent. Down regulation of COX-2 expression in rabbit articular chondrocytes in a dose (0–100μg) and time (0–48h) dependent manner, unveiled its in vitro anti-inflammatory significance. In vivo carrageenan induced inflammatory rat model demonstrated a 68.19% inhibition of inflammation whereas an inflammation inhibition potential of 79.38% was recorded in anti-arthritic complete Freund’s adjuvant-induced arthritic rat model. A substantial ameliorating effect on altered hematological and biochemical parameters in arthritic rats was also observed. Therefore, findings of the present study prospects fucoidan as a potential antioxidant that can effectively abrogate oxidative stress, edema and arthritis-mediated inflammation and mechanistic studies are recommended for observed activities.