Comparative study on antifungal activities of chitosan nanoparticles and chitosan silver nano composites against Fusarium oxysporum species complex

• Published in: International journal of biological macromolecules 2017-12, Vol.105 (Pt 1), p.478-488
• Main Authors: Dananjaya, S.H.S., Erandani, W.K.C.U., Kim, Cheol-Hee, Nikapitiya, Chamilani, Lee, Jehee, De Zoysa, Mahanama
• Format: Article
• Language: English
• Subjects: Animals; Antifungal; Antifungal Agents - chemistry; Antifungal Agents - pharmacology; Antifungal Agents - toxicity; Cell Line; Cell Survival - drug effects; Chitosan - chemistry; Chitosan - pharmacology; Chitosan - toxicity; Chitosan nanoparticles; Chitosan silver nano- composites; F. oxysporum; Fusarium - drug effects; Fusarium - growth & development; Fusarium - metabolism; Humans; Metal Nanoparticles - chemistry; Mycelium - drug effects; Mycelium - growth & development; Particle Size; Reactive Oxygen Species - metabolism; Silver - chemistry; Zebrafish - microbiology; Zeta potential
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2017.07.056

[Display omitted] Though the metal nanoparticles (NPs) have been shown favorable results against fungal diseases, erratic environmental toxicity of NPs have raised serious concerns against their applications. Hence, it is vital to modify antifungal compounds into safe substitutes over synthetic chemicals. In this study, antifungal effects of chitosan nanoparticles (CNPs) and chitosan silver nanocomposites (CAgNCs) were compared against Fusarium oxysporum species complex. CNPs and CAgNCs were synthesized, characterized and compared based on the transmission electron microscope, X-ray diffraction, UV–vis absorbance spectra, particle size distribution, zeta potential and thermal stability analysis. Ultra-structural analysis on mycelium membrane of treated F. oxysporum showed that CNPs and CAgNCs could induce a pronounced membrane damage and disruption of the mycelium surface, increase the membrane permeability, and even cell disintegration. CAgNCs showed a significantly higher radial growth inhibition than CNPs in all the tested concentrations. Both CNPs and CAgNCs were not only effective in reducing the fungal growth, but also caused morphological and ultrastructural changes in the pathogen, thereby suggesting its usage as an antifungal dispersion system to control F. oxysporum. Additionally, CNPs and CAgNCs therapy reduced the F. oxysporum infection in zebrafish. Data demonstrates biologically active CNPs and CAgNCs are promising antifungal agents against F. oxysporum.