Biocompatiable silk fibroin/carboxymethyl chitosan/strontium substituted hydroxyapatite/cellulose nanocrystal composite scaffolds for bone tissue engineering

Bone defects arise from trauma, skeletal diseases or tumor resections have become a critical clinical challenge. Biocomposite materials as artificial bone repair materials provide a promising approach for bone regeneration. In this study, we used silk fibroin (SF), carboxymethyl chitosan (CMCS), cel...

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Bibliographic Details
Published in:International journal of biological macromolecules 2019-09, Vol.136, p.1247-1257
Main Authors: Zhang, Xiao-yun, Chen, Yue-ping, Han, Jie, Mo, Jian, Dong, Pan-feng, Zhuo, Ying-hong, Feng, Yang
Format: Article
Language:English
Subjects:
Biocompatible Materials - chemistry
Biocompatible Materials - pharmacology
Bone and Bones - cytology
Bone repair scaffold
Bone tissue engineering
Cell Survival - drug effects
Cellulose - chemistry
Cellulose nanocrystal
Chitosan - analogs & derivatives
Chitosan - chemistry
Fibroins - chemistry
Humans
Hydroxyapatites - chemistry
Materials Testing
Nanocomposites - chemistry
Nanoparticles - chemistry
Osteogenesis - drug effects
Silk fibroin
Strontium - chemistry
Strontium substituted hydroxyapatite
Tissue Engineering
Tissue Scaffolds - chemistry
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Summary:Bone defects arise from trauma, skeletal diseases or tumor resections have become a critical clinical challenge. Biocomposite materials as artificial bone repair materials provide a promising approach for bone regeneration. In this study, we used silk fibroin (SF), carboxymethyl chitosan (CMCS), cellulose nanocrystals (CNCs) and strontium substituted hydroxyapatite (Sr-HAp) to prepare the biocomposite scaffolds of SF/CMCS, SF/CMCS/CNCs, SF/CMCS/CNCs/Sr-HAp. The characterization results showed that all the SF-based scaffolds have a porous sponge-like structure with porosities over 80%. In addition, there was a significant increase in compressive strength of SF/CMCS/Sr-HAp/CNCs scaffold when compared to that of SF/CMCS scaffolds, while maintaining high porosity with lower swelling ratio. All the SF-based scaffolds were non-toxic and had a good hemocompatibility. Comparing to the SF/CMCS scaffold, the scaffolds with addition of Sr-HAp and/or CNCs showed enhanced protein adsorption and ALP activity. In addition, higher expression of osteogenic gene markers such as RUNX2, ALP, OCN, OPN, BSP and COL-1 further substantiated the applicability of SF/CMCS/Sr-HAp/CNCs scaffolds for bone related applications. Hence, this study suggests that SF/CMCS/Sr-HAp/CNCs scaffolds have a potential in non-loading bone repair application.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2019.06.172