Exploring the influence of brilliant blue G on amyloid fibril formation of lysozyme

Evidence suggests that amyloid fibril mitigation/inhibition is considered a promising approach toward treating amyloid diseases. In this work, we first examined how amyloid fibrillogenesis of lysozyme was affected by BBG, a safe triphenylmethane compound with nice blood–brain-barrier-permeability, a...

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Bibliographic Details
Published in:International journal of biological macromolecules 2019-10, Vol.138, p.37-48
Main Authors: How, Su-Chun, Hsin, Ai, Chen, Guan-Yu, Hsu, Wei-Tse, Yang, Szu-Ming, Chou, Wei-Lung, Chou, Shiu-Huey, Wang, Steven S.-S.
Format: Article
Language:English
Subjects:
Amyloid - chemistry
Amyloid - toxicity
Binding Sites
Cell Line, Tumor
Cell Survival - drug effects
Humans
Molecular Docking Simulation
Muramidase - chemistry
Muramidase - toxicity
Protein Aggregates - drug effects
Protein Conformation
Rosaniline Dyes - pharmacology
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Summary:Evidence suggests that amyloid fibril mitigation/inhibition is considered a promising approach toward treating amyloid diseases. In this work, we first examined how amyloid fibrillogenesis of lysozyme was affected by BBG, a safe triphenylmethane compound with nice blood–brain-barrier-permeability, and found that shorter fibrillar species were formed in the lysozyme samples treated with BBG. Next, alterations in the features including the secondary as well as tertiary structure, extent of aggregation, and molecular distribution of lysozyme triggered by the addition of BBG were examined by various spectroscopic techniques, right-angle light scattering, dynamic light scattering, and SDS-PAGE. In addition, we have investigated how BBG affected the lysozyme fibril-induced cytotoxicity in SH-SY5Y cells. We found that a large quantity of shorter fibrillar species and more lysozyme monomers were present in the samples treated with BBG. Also, the addition of BBG rescued SH-SY5Y cells from cell death induced by amyloid fibrils of lysozyme. Finally, information about the binding sites and interacting forces involved in the BBG-lysozyme interaction was further explored using synchronous fluorescence and molecular docking approaches. Molecular docking results revealed that, apart from the hydrophobic interaction(s), hydrogen bonding, electrostatic interactions, and van der Waal forces may also be involved in the binding interaction.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2019.07.055