Thyroxine-loaded chitosan/carboxymethyl cellulose/hydroxyapatite hydrogels enhance angiogenesis in in-ovo experiments

[Display omitted] Angiogenesis is one of the most important processes in repair and regeneration of many tissues and organs. Blood vessel formation also play a major role in repair of dental tissue(s) after ailments like periodontitis. Here we report the preparation of chitosan/carboxymethyl cellulo...

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Bibliographic Details
Published in:International journal of biological macromolecules 2020-02, Vol.145, p.1162-1170
Main Authors: Malik, Muhammad Hamza, Shahzadi, Lubna, Batool, Razia, Safi, Sher Zaman, Khan, Abdul Samad, Khan, Ather Farooq, Chaudhry, Aqif Anwar, Rehman, Ihtesham Ur, Yar, Muhammad
Format: Article
Language:English
Subjects:
Alveolar Bone Loss - therapy
Alveolar Process
Angiogenesis
Angiogenesis Inducing Agents - pharmacology
Animals
Carboxymethyl cellulose
Carboxymethylcellulose Sodium - chemistry
Carboxymethylcellulose Sodium - pharmacology
Cell Adhesion
Cell Proliferation
Cellulose
Chickens
Chitosan
Chitosan - analogs & derivatives
Chitosan - chemistry
Chitosan - pharmacology
Chorioallantoic Membrane
Drug Liberation
Durapatite - chemistry
Durapatite - pharmacology
Hydrogels - chemistry
Hydrogels - pharmacology
Hydroxyapatite
Injectable gels
Periodontal regeneration
Thyroxine
Thyroxine - chemistry
Thyroxine - pharmacology
Tissue engineering
Tissue Engineering - methods
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Summary:[Display omitted] Angiogenesis is one of the most important processes in repair and regeneration of many tissues and organs. Blood vessel formation also play a major role in repair of dental tissue(s) after ailments like periodontitis. Here we report the preparation of chitosan/carboxymethyl cellulose/hydroxyapatite based hydrogels, loaded with variable concentrations of thyroxin i.e., 0.1 μg/ml, 0.5 μg/ml and 1 μg/ml. Scanning electron microcopy images (SEM) showed all hydrogels were found to be porous and solution absorption study exhibited high swelling potential in aqueous media. FTIR spectra confirmed that the used materials did not change their chemical identity in synthesized hydrogels. The synthesized hydrogels demonstrated good bending, folding, rolling and stretching abilities. The hydrogels were tested in chick chorioallantoic membrane (CAM) assay to investigate their angiogenic potential. Hydrogel containing 0.1 μg/ml of thyroxine showed maximum neovascularization. For cytotoxicity analyses, preosteoblast cells (MC3T3-E1) were seeded on these hydrogels and materials were found to be non-toxic. These hydrogels with pro-angiogenic activity possess great potential to be used for periodontal regeneration.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2019.10.043