Structural characterization and antioxidant effect of green alga Enteromorpha prolifera polysaccharide in Caenorhabditis elegans via modulation of microRNAs

• Published in: International journal of biological macromolecules 2020-05, Vol.150, p.1084-1092
• Main Authors: Lin, Guo-peng, Wu, De-sheng, Xiao, Xiao-wei, Huang, Qian-yun, Chen, Hong-bin, Liu, Dan, Fu, Hai-qing, Chen, Xin-hua, Zhao, Chao
• Format: Article
• Language: English
• Subjects: Animals; Antioxidant; Antioxidants - chemistry; Antioxidants - pharmacology; Caenorhabditis elegans; Caenorhabditis elegans - genetics; Caenorhabditis elegans - metabolism; Chlorophyta - chemistry; Enteromorpha prolifera; Gene Expression Regulation, Plant - drug effects; Gene Expression Regulation, Plant - radiation effects; MicroRNAs; MicroRNAs - biosynthesis; MicroRNAs - genetics; Oxidative Stress - drug effects; Oxidative Stress - radiation effects; Polysaccharide; Polysaccharides - chemistry; Polysaccharides - pharmacology; RNA, Plant - biosynthesis; RNA, Plant - genetics; Ultraviolet Rays
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2019.10.114

A water-soluble heteropolysaccharide was isolated and purified from Enteromorpha prolifera by DEAE-52 and Bio-Gel P-2 column chromatography. Fourier transform infrared spectroscopy (FTIR), high performance liquid chromatography (HPLC), multi-angle laser light scattering (MALLS), and nuclear magnetic resonance (NMR) spectroscopy were used to characterize the structure of E. prolifera polysaccharide degradation (EPP-1). Its anti-oxidative activity was determined in Caenorhabditis elegans via modulation of microRNAs. The average molecular weight of EPP-1 was 4.28 kDa. It contained six types of linkage units as →2)-β-d-GlcpA-(1→, →3,6)-β-d-Manp-(1→, →4)-α-d-Glcp-(1→, →6)-β-d-Galp-(1→, β-l-Rhap-(1→, and →4)-β-d-GalpA-(1→. The mean lifespan, ultraviolet-induced oxidative stress, and thermotolerance in C. elegans were improved after treatment of EPP-1. Moreover, EPP-1 significantly increased the total superoxide dismutase levels and decreased the malondialdehyde levels in C. elegans. Intracellular reactive oxygen species accumulation and DNA damage were ameliorated by up-regulation of SKN-1 and DAF-16 expression through miR-48 and miR-51 miR-186 down-regulation. In vivo studies demonstrated that EPP-1 might be applied in functional foods as the antioxidative and anti-ageing ingredient.