Glucose oxidase-copper hybrid nanoflowers embedded with magnetic nanoparticles as an effective antibacterial agent

• Published in: International journal of biological macromolecules 2020-07, Vol.155, p.1520-1531
• Main Authors: Lee, Inseon, Cheon, Hong Jae, Adhikari, Manab Deb, Tran, Tai Duc, Yeon, Kyung-Min, Kim, Moon Il, Kim, Jungbae
• Format: Article
• Language: English
• Subjects: Amines - chemistry; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Antibacterial activity; Biocatalysis; Copper - chemistry; Enzyme-inorganic hybrid nanoflowers; Escherichia coli - drug effects; Glucose oxidase; Glucose Oxidase - metabolism; Hydrogen-Ion Concentration; Magnetic Iron Oxide Nanoparticles - chemistry; Oxidation-Reduction; Staphylococcus aureus - drug effects
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2019.11.129

Bacterial contamination causes various problems ranging from bacterial infection to biofouling. As an effective and non-toxic agent for bacterial de-contamination, glucose oxidase (GOx)-copper hybrid nanoflowers embedded with amine-functionalized magnetic nanoparticles (NH2-MNPs), called ‘MNP-GOx NFs’, are developed. Positively-charged NH2-MNPs and negatively-charged GOx molecules are first interacted via electrostatic attraction which can be controlled by changing the buffer pH, and the follow-up addition of copper(II) sulfate leads to blooming of nanoflowers (MNP-GOx NFs) after incubation at room temperature for 3 days. MNP-GOx NFs show effective antibacterial activity by generating H2O2 from GOx-catalyzed glucose oxidation. For example, 99.9% killings of Staphylococcus aureus and Escherichia coli are achieved after 3 h treatment of 106/mL cells with 0.2 and 3.0 mg/mL MNP-GOx NFs, respectively, revealing that Gram-positive S. aureus with mono-layer membrane system is more vulnerable to the treatment of MNP-GOx NFs than Gram-negative E. coli with two-layer membrane system. MNP-GOx NFs can maintain 97% of bactericidal activity even after recycled uses by magnetic separation for eight times iterative bacterial killings. Finally, MNP-GOx NFs are employed for the fabrication of antibacterial gauzes. MNP-GOx NFs have also opened up a great potential for their applications in biosensors, biofuel cells and bioconversion as well as bacterial de-contamination. •Magnetic nanoparticles and glucose oxidase are complexed via charge interaction.•Follow-up addition of CuSO4 leads to the blooming of hybrid nanoflowers (MNP-GOx NFs).•MNP-GOx NFs show antibacterial activity against S. aureus and E. coli.•MNP-GOx NFs maintain 97% of bactericidal activity after recycled uses eight times.•MNP-GOx NFs are employed to develop antibacterial gauzes.