Quantitative insight into dispersity and antibactericidal capability of silver nanoparticles noncovalently conjugated by polysaccharide-protein complexes

• Published in: International journal of biological macromolecules 2020-05, Vol.150, p.459-467
• Main Authors: Jian, Wenjie, Ma, Ying, Zhu, Xiaopei, Zhang, Ni, Lin, Lin, Jia, Binmei, Shen, Xiulin, Xiong, Hejian, Wang, Wenying
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Antibacterial activities; Bacteria - drug effects; Dispersibility; Dynamic light scattering; Environment; Environmental factors; Hydrodynamics; Hydrogen-Ion Concentration; Metal Nanoparticles - chemistry; Metal Nanoparticles - ultrastructure; Microbial Sensitivity Tests; Multiple-light-scattering; Polysaccharides - chemistry; Proteins - chemistry; Silver - chemistry; Silver nanoparticles; Temperature
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2020.02.098

Precise prediction and measurement of dispersibility of silver nanoparticles (AgNPs) under atmospheric conditions are extremely vital for their potential commercial application. In the present work, the dispersibility of AgNPs capped by polysaccharide-protein from viscera of abalone (PSP-AgNPs) was studied using the combination of ultraviolet–visible spectroscopy (UV–vis), dynamic light scattering (DLS) and multiple-light-scattering (MLS) techniques. The results showed that the combination of UV/vis, DLS and MLS not only accurately determined the dispersibility of PSP-AgNPs, but also provided detailed information about the aggregation behavior of PSP-AgNPs. Furthermore, the results revealed a high dispersibility of PSP-AgNPs in the studied environment. The system temperature, pH value and thermal treatment (pasteurization and sterilization) had no effect on the dispersion of PSP-AgNPs in the effective concentration range against the pathogenic bacteria. Besides, an excellent stable dispersion and antibacterial activity against common pathogenic vibrio was also found in the dispersed PSP-AgNPs in seawater. Overall, the study provides a suitable method for the precise measurement of the dispersibility of AgNPs in environment. The AgNPs act as a potential bactericide with good dispersion and antibacterial activity in mariculture and other fields.