Preparation and characterization of green carboxymethylchitosan (CMCS) – Polyvinyl alcohol (PVA) electrospun nanofibers containing gold nanoparticles (AuNPs) and its potential use as biomaterials

• Published in: International journal of biological macromolecules 2020-05, Vol.151, p.821-829
• Main Authors: Ibrahim, H.M., Reda, Maysa M., Klingner, A.
• Format: Article
• Language: English
• Subjects: Antibacterial-biological application; Biocompatible Materials - chemistry; Carboxymethylchitosan nanofibers; Cell Line; Cell Survival; Chitosan - analogs & derivatives; Chitosan - chemistry; Gold; Gold nanoparticles; Humans; Magnetic Resonance Spectroscopy; Metal Nanoparticles - chemistry; Metal Nanoparticles - ultrastructure; Molecular Structure; Nanofibers - chemistry; Nanofibers - ultrastructure; Polyvinyl Alcohol - chemistry; Spectroscopy, Fourier Transform Infrared; Spectrum Analysis
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2020.02.174

Green chemistry was used in nanostructures preparation to impart it amazing innovating application in the medical field. Herein we prepared novel, cost effective and ultra-safe antibacterial nanocomposite. This nanocomposite contains carboxymethylchitosan (CMCS) as safe reducing agent for gold nanoparticles (AuNPs) and polyvinyl alcohol (PVA) as nanofiber aiding material formation. The AuNPs has spherical shapes, its diameter ranged from 15 to 25 nm and uniform distributed within CMCS nanofibers. The optimum conditions for electrospinning were 10 wt% total solution contains 2 wt% CMCS and 8 wt% PVA. UV–vis, TEM and XRD were used to characterize AuNPs whereas FTIR and SEM were used to characterize nanofibers. Results showed that ultra-fine fibers were generated after addition of PVA to CMCS solution. Also, CMCS nanofibers containing AuNPs has excellent antibacterial activity towards tested bacteria. Finally, the cytotoxicity of CMCS has been evaluated through Cell viability assay, which confirm that the nanofiber composite is non-toxic and tissue compatible. •Green nanocomposites contain carboxymethylchitosan (CMCS) nanofibers, gold nanoparticles (AuNPs) and poly vinyl alcohol (PVA) were prepared.•The nanocomposite has higher antibacterial activity with minimal toxicity•The AuNPs has spherical shapes and its diameter ranged from 15 to 25 nm and uniformly distributed within the prepared nanofibers.•The higher antibacterial activity and lower cytotoxicity of this nanocomposite introduce it to be used in biomedical applications.