Hemostatic, biocompatible, and antibacterial non-animal fungal mushroom-based carboxymethyl chitosan-ZnO nanocomposite for wound-healing applications

In this study, non-animal mushroom carboxymethyl chitosan (NAM-CMCS) was used as a natural polymer stabilizing agent in the ultrasonic preparation of a ZnO nanocomposite at ambient laboratory temperatures. The formation and morphology of the ZnO nanoparticles were investigated by applying FTIR, XRD,...

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Bibliographic Details
Published in:International journal of biological macromolecules 2020-07, Vol.155, p.71-80
Main Authors: Rao, Kummara Madhusudana, Suneetha, Maduru, Park, Gyu Tae, Babu, Anam Giridhar, Han, Sung Soo
Format: Article
Language:English
Subjects:
Agaricales - chemistry
Animals
Anti-Bacterial Agents - chemistry
Anti-Bacterial Agents - pharmacology
Antibacterial
Bandages
Biocompatible
Carboxymethyl chitosan
Cell Line
Chitosan - analogs & derivatives
Chitosan - chemistry
Chitosan - pharmacology
Escherichia coli - drug effects
Hemostatic
Hemostatics - chemistry
Hemostatics - pharmacology
Humans
Nanoparticles - chemistry
Staphylococcus aureus - drug effects
Swine
Wound dressing
Wound Healing - drug effects
Zinc Oxide - chemistry
Zinc Oxide - pharmacology
ZnO
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Summary:In this study, non-animal mushroom carboxymethyl chitosan (NAM-CMCS) was used as a natural polymer stabilizing agent in the ultrasonic preparation of a ZnO nanocomposite at ambient laboratory temperatures. The formation and morphology of the ZnO nanoparticles were investigated by applying FTIR, XRD, XPS, FE-SEM, and DLS techniques. The FTIR and XPS spectra confirmed the presence of NAM-CMCS functional groups and ZnO in the nanoparticles. The prepared NAM-CMCS-ZnO nanoparticles were shown by FE-SEM to have a spherical shape and an average diameter of 18 ± 3.6 nm. The DLS-determined size distribution showed the NAM-CMCS-ZnO nanoparticle size averaged 21 ± 2.9 nm. Finally, cytocompatibility, hemostasis, and antibacterial performance were assessed in vitro to evaluate the biological performance of NAM-CMCS-ZnO nanoparticles. In vitro Prestoblue® assay and live/dead test results from skin fibroblast and keratinocytes confirmed the developed NAM-CMCS-ZnO nanoparticles were biocompatible over a wide range of concentrations (0–500 μg/well). The NAM-CMCS-ZnO nanoparticles exhibited synergetic antibacterial properties against Gram-positive (Staphylococcus aureus) bacteria. Moreover, the nanoparticles showed hemostatic properties with good hemocompatibility. The overall excellent biological properties of NAM-CMCS-ZnO nanoparticles indicate its suitability for use in wound dressing applications.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2020.03.170