Callophycin A loaded chitosan and spicules based nanocomposites as an alternative strategy to overcome vaginal candidiasis

• Published in: International journal of biological macromolecules 2020-10, Vol.161, p.656-665
• Main Authors: Arumugam, Ganeshkumar, Rajendran, Rajaram
• Format: Article
• Language: English
• Subjects: Animals; Candidiasis, Vulvovaginal - drug therapy; Candidiasis, Vulvovaginal - metabolism; Candidiasis, Vulvovaginal - pathology; Carbolines - chemistry; Carbolines - pharmacology; Chitosan; Chitosan - chemistry; Chitosan - pharmacology; Disease Models, Animal; Drug delivery; Female; Histo-morphology; Humans; Marine natural product; Mice; Nanocomposites - chemistry; Nanocomposites - therapeutic use; Pro- inflammatory; Vaginal candidiasis
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2020.06.119

The present study aimed to understand the killing effects of seaweed derived metabolite Callophycin A (Cal A). In vitro studies confirmed that the beneficial effects of Cal A on the viability of C. albicans. To enhance the biological activity, we used to demonstrated that chitosan and spicules as a drug carrier. The Callophycin A loading was confirmed by spectral variation of FT-IR and morphological variation by SEM. Moreover, around 65% and 38% of Cal A was successfully loaded in chitosan and spicules respectively. Further, VVC induced animal model experiments confirmed that the candidicidal activity of 1% clotrimazole, Cal A, Cal@Chi and Cal@Spi. After 6 days of treatment Cal@Chi produces a significant reduction in the fungal burden of vaginal lavage. The histo-morphological alterations also evidenced that the protective role of Cal@Chi in VVC model. The present investigations are known to be the first and foremost study to discriminate the potentiality of Cal A composites. Cal A loaded chitosan nanoparticles could be used as an alternative strategy for the development of the novel marine natural product based topical applications. •Sponge derived spicules were successfully isolated and characterised.•The in-vitro anti-candidal potential of different forms of Callophycin A evaluated using different C .albicans.•In-vivo experiments confirmed that the role of forms of Callophycin A on protecting Candida infection.•1% clotrimazole and 1% Cal@Chi showed good results.