Effects of WSG, a polysaccharide from Ganoderma lucidum, on suppressing cell growth and mobility of lung cancer

WSG is a water soluble polysaccharides isolated from Ganoderma lucidum. In this study, we showed that WSG, a glucose-rich polysaccharide with an average molecular mass of approximately 1000 kDa, effectively inhibited cell viability and mobility of lung cancer cells. Functional studies revealed that...

Full description

Saved in:
Bibliographic Details
Published in:International journal of biological macromolecules 2020-12, Vol.165 (Pt A), p.1604-1613
Main Authors: Hsu, Wei-Hung, Qiu, Wei-Lun, Tsao, Shu-Ming, Tseng, Ai-Jung, Lu, Mei-Kuang, Hua, Wei-Jyun, Cheng, Hsin-Chung, Hsu, Hsien-Yeh, Lin, Tung-Yi
Format: Article
Language:English
Subjects:
Animals
Cell Cycle - drug effects
Cell Line, Tumor
Cell Proliferation - drug effects
Degradation
EGFR
Ganoderma lucidum
Gene Expression Regulation, Neoplastic - drug effects
Humans
Lung cancer
Lung Neoplasms - drug therapy
Lung Neoplasms - pathology
MAP Kinase Signaling System - drug effects
Mice
Neoplasm Proteins - genetics
Polysaccharides
Polysaccharides - chemistry
Polysaccharides - pharmacology
Reishi - chemistry
TGFβR
Xenograft Model Antitumor Assays
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:WSG is a water soluble polysaccharides isolated from Ganoderma lucidum. In this study, we showed that WSG, a glucose-rich polysaccharide with an average molecular mass of approximately 1000 kDa, effectively inhibited cell viability and mobility of lung cancer cells. Functional studies revealed that WSG reduced phosphorylation of ERK1/2 in cells upon either EGF or TGFβ stimulation. WSG also inhibited phosphorylation of multiple intracellular signaling molecules such as FAK, AKT and Smad2. Mechanistically, we demonstrated that WSG induced degradation of TGFβ and EGF receptors via proteasome and lysosome, respectively. Moreover, we found that WSG significantly suppressed lung tumor growth, reduced the size of metastatic nodules in the lungs and prolonged the survival of LLC1-bearing mice. Our findings suggested that WSG may have potential as a therapeutic intervention for treatment of lung cancer. •WSG inhibited tumor growth and prolonged the survival of LLC1-bearing mice.•WSG inhibited cell viability and mobility of lung cancer cells.•WSG induced protein degradation of EGFR and TGFβRs.•Simultaneous inhibition of EGFR and TGFβRs enhances cytotoxicity.
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2020.09.227