Antioxidant and antibacterial polyelectrolyte wound dressing based on chitosan/hyaluronan/phosphatidylcholine dihydroquercetin

• Published in: International journal of biological macromolecules 2021-01, Vol.166, p.18-31
• Main Authors: Hassan, Mohamed A., Tamer, Tamer M., Valachová, Katarína, Omer, Ahmed M., El-Shafeey, Muhammad, Mohy Eldin, Mohamed S., Šoltés, Ladislav
• Format: Article
• Language: English
• Subjects: Animals; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Anti-Inflammatory Agents; Antioxidant; Antioxidants - chemistry; Antioxidants - pharmacology; Bandages; Cells, Cultured; Chitosan; Chitosan - analogs & derivatives; Erythrocytes - drug effects; Female; Hemolysis; Humans; Hyaluronan; Hyaluronic Acid - analogs & derivatives; Mice; NIH 3T3 Cells; Phosphatidylcholine dihydroquercetin (PCDQ); Phosphatidylcholines - chemistry; Quercetin - analogs & derivatives; Quercetin - chemistry; Quercetin - pharmacology; Rats; Rats, Wistar; Re-Epithelialization - drug effects; Wound healing
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2020.11.119

Antioxidant and antimicrobial wound dressings are the most favorable for acute and chronic wounds treatment. Herein, we formulated a multifunctional polyelectrolyte wound dressing membrane on the basis of chitosan (Ch) and hyaluronan (HA) enhanced by phosphatidylcholine dihydroquercetin (PCDQ). Physicochemical properties and microstructures of fabricated films were investigated adopting Fourier transform infrared (FT-IR) spectroscopy, thermogravimetric analysis (TGA) and scanning electron microscope (SEM). Furthermore, water uptakes, wettability profiles, surface roughness, and mechanical characteristics of the developed membranes were studied. The developed wound dressing revealed free radical scavenging potency, hemocompatibility with a tendency to enhance blood clotting. Furthermore, incorporation of PCDQ significantly promoted the antibacterial and anti-inflammatory activities of Ch/HA/PCDQ. Moreover, Ch/HA/PCDQ films exhibited cellular compatibility towards mouse fibroblast cells. The capability of Ch/HA/PCDQ to promote wound healing was evaluated using adult Wistar albino female rats. The in vivo findings demonstrated that Ch/HA/PCDQ films significantly ameliorated mouse full-thickness wounds as evidenced by a reduction in the wound area. Moreover, histological examinations of wounds dressed with Ch/HA/PCDQ illustrated a prominent re-epithelialization compared with wounds handled with the cotton gauze and Ch/HA dressings, exposing the efficiency of PCDQ. These findings emphasized that a Ch/HA/PCDQ membrane has outstanding potential for wound healing and skin regeneration. •A novel chitosan/hyaluronan/phosphatidylcholine dihydroquercetin membrane was fabricated and characterized.•The Ch/HA/PCDQ dressing demonstrated antioxidant, antibacterial and anti-inflammatory competency.•The developed Ch/HA/PCDQ membrane exposed salient hemocompatibility and cytocompatibility.•A significant improvement in the wound healing was perceived by Ch/HA/PCDQ dressing.