Disruption of the cytochrome P450 CYP6BQ7 gene reduces tolerance to plant toxicants in the red flour beetle, Tribolium castaneum

• Published in: International journal of biological macromolecules 2021-03, Vol.172, p.263-269
• Main Authors: Zhang, Yuan-chen, Gao, Shan-shan, Xue, Shuang, An, Shi-heng, Zhang, Kun-peng
• Format: Article
• Language: English
• Subjects: Animals; Artemisia - chemistry; Artemisia - metabolism; Brain - drug effects; Brain - metabolism; Cytochrome P-450 Enzyme System - genetics; Cytochrome P-450 Enzyme System - metabolism; Cytochrome P450; Essential oil; Female; Gene Expression Regulation; Hemolymph - drug effects; Hemolymph - metabolism; Insecticides - isolation & purification; Insecticides - metabolism; Insecticides - pharmacology; Larva - drug effects; Larva - genetics; Larva - growth & development; Larva - metabolism; Longevity - drug effects; Longevity - genetics; Male; Mortality rate; Oils, Volatile - isolation & purification; Oils, Volatile - metabolism; Oils, Volatile - pharmacology; Plant Proteins - antagonists & inhibitors; Plant Proteins - genetics; Plant Proteins - metabolism; Pupa - drug effects; Pupa - genetics; Pupa - growth & development; Pupa - metabolism; RNA interference; RNA, Small Interfering - genetics; RNA, Small Interfering - metabolism; Tribolium - drug effects; Tribolium - genetics; Tribolium - growth & development; Tribolium - metabolism; Tribolium castaneum
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2021.01.054

In insects, the cytochrome P450 CYP6B family plays key roles in the detoxification of toxic plant substances. However, the function of CYP6 family genes in degrading plant toxicants in Tribolium castaneum, an extremely destructive global storage pest, have yet to be elucidated. In this study, a T. castaneum CYP gene, TcCYP6BQ7, was characterized. TcCYP6BQ7 expression was significantly induced after exposure to essential oil of the plant Artemisia vulgaris (EOAV). Spatiotemporal expression profiling revealed that TcCYP6BQ7 expression was higher in larval and adult stages of T. castaneum than in other developmental stages, and that TcCYP6BQ7 was predominantly expressed in the brain and hemolymph from the late larval stage. TcCYP6BQ7 silencing by RNA interference increased larvae mortality in response to EOAV from 49.67% to 71.67%, suggesting that this gene is associated with plant toxicant detoxification. Combined results from this study indicate that the CYP6 family gene TcCYP6BQ7 likely plays a pivotal role in influencing the susceptibility of T. castaneum to plant toxicants. These findings may have implications for the development of novel therapeutics to control this agriculturally important pest.