Electrospun PLGA/SF/artemisinin composite nanofibrous membranes for wound dressing

• Published in: International journal of biological macromolecules 2021-07, Vol.183, p.68-78
• Main Authors: Peng, Yan, Ma, Yan, Bao, Yu, Liu, Zulan, Chen, Li, Dai, Fangyin, Li, Zhi
• Format: Article
• Language: English
• Subjects: Animals; Anti-Infective Agents - chemistry; Anti-Infective Agents - pharmacology; Anti-inflammatory; Artemisinin; Artemisinins - chemistry; Artemisinins - pharmacology; Bandages; Cytokines - metabolism; Delayed-Action Preparations; Disease Models, Animal; Drug Carriers; Drug Compounding; Drug Liberation; Fibroins - chemistry; Macrophages - drug effects; Macrophages - metabolism; Male; Membranes, Artificial; Mice; Nanofiber; Nanofibers; Polylactic Acid-Polyglycolic Acid Copolymer - chemistry; Rats; Rats, Sprague-Dawley; RAW 264.7 Cells; Skin - drug effects; Skin - injuries; Skin - metabolism; Skin - pathology; Wound dressing; Wound Healing - drug effects; Wounds, Penetrating - drug therapy; Wounds, Penetrating - metabolism; Wounds, Penetrating - pathology
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2021.04.021

Combining biodegradable materials with natural plant components for wound dressing has been receiving significant attention. ART is a sesquiterpene lactone compound extracted from Artemisia annua L., possessing multiple pharmacological effects including antibacterial activity and anti-inflammatory property. Herein, the blended polylactic acid glycolic acid (PLGA)/silk fibroin (SF) membranes loaded with artemisinin (ART) are fabricated through electrospinning. With aid of SF, the fabricated membranes have a good sustained-release effect, and the accumulated ART release can reach 69% after three weeks. PLGA/SF/ART membranes exhibit favorable anti-inflammatory and cell compatibility in vitro evaluations. The in vivo experiment indicates that PLGA/SF/ART2 membranes can shorten the inflammation period and enhance skin regeneration in a full-thickness wound model through down-regulating the expressions of pro-inflammatory cytokines IL-1β and TNF-α. To sum up, the fabricated PLGA/SF/ART2 composite membranes with anti-inflammatory properties can be a proposal wound dressing for chronic wound healing. •Artemisinin was successfully loaded on membranes as an anti-inflammatory agent.•Therapeutic effect was analyzed from the level of inflammatory factors•The fabricated membrane shows anti-inflammatory property without cytotoxicity.•This material has a good potential for using as a wound dressing.