Synergistic effect of lotus seed resistant starch and short-chain fatty acids on mice fecal microbiota in vitro

This study aimed to investigate the synergistic effect of lotus seed resistant starch (LRS) and short-chain fatty acids (SCFAs) on mice fecal bacterial flora and the contents of SCFAs in vitro. Following 24 h of fermentation, 16S rRNA analysis revealed several differences in the fecal microbiota com...

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Bibliographic Details
Published in:International journal of biological macromolecules 2021-07, Vol.183, p.2272-2281
Main Authors: Li, Xin, Lei, Suzhen, Liu, Lu, Zhang, Yi, Zheng, Baodong, Zeng, Hongliang
Format: Article
Language:English
Subjects:
Animals
Bacteria - drug effects
Bacteria - genetics
Bacteria - growth & development
Bacteria - metabolism
Butyrate
Drug Synergism
Fatty Acids, Volatile - isolation & purification
Fatty Acids, Volatile - pharmacology
Fecal microbiota
Feces - microbiology
Fermentation - drug effects
Gastrointestinal Microbiome - drug effects
Lotus - chemistry
Lotus seed resistant starch
Male
Mice
Mice, Inbred ICR
Prebiotics
Resistant Starch - pharmacology
Ribotyping
Seeds - chemistry
Short-chain fatty acids
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Summary:This study aimed to investigate the synergistic effect of lotus seed resistant starch (LRS) and short-chain fatty acids (SCFAs) on mice fecal bacterial flora and the contents of SCFAs in vitro. Following 24 h of fermentation, 16S rRNA analysis revealed several differences in the fecal microbiota community structure among primal bacteria (PB), LRS and different SCFAs combined with LRS groups (SCFAs-LRS). The LRS group increased the relative abundance of Lactobacillus, Allobaculum, Clostridium, Bacteroides and Prevotella. Among the SCFAs-LRS group, AA-LRS increased the relative abundance of Prevotella, and Bacillus. PA-LRS increased abundance of Sphingomonas and the BA-LRS group significantly increased the relative abundance of Rhizobiales, Brucellaceae and Ochrobactrum. Meanwhile, propionic acid and BA productions significantly increased in the BA-LRS group. The SCFAs-LRS group elicited a beneficial effect on the fecal microbiota by increasing production of SCFAs. We highlight the fact that the combination of LRS and SCFA can increase the contents of SCFAs produced by mice fecal microbiota. In short, the combination of LRS and SCFA can influence intestinal flora by promoting the growth of beneficial bacteria and can serve as new prebiotics for promoting health and disease management. •BA-LRS group promoted fecal microbial diversity.•SCFA-LRS significantly increased the levels of Bacteroidetes (P 
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2021.06.016