Biohydrogen production by immobilized Enterobacter aerogenes on functionalized multi-walled carbon nanotube

• Published in: International journal of hydrogen energy 2019-05, Vol.44 (28), p.14395-14405
• Main Authors: Boshagh, Fatemeh, Rostami, Khosrow, Moazami, Nasrin
• Format: Article
• Language: English
• Subjects: Biohydrogen production; Carbon nanotube; Dark fermentation; Enterobacter aerogenes; Immobilization
• ISSN: 0360-3199; 1879-3487
• DOI: 10.1016/j.ijhydene.2018.11.199

Hydrogen production by immobilized Enterobacter aerogenes on functionalized multi-walled carbon nanotube (MWCNT-COOH) in repeated batch mode was studied. Fourier transform infrared (FTIR) spectroscopy and field emission scanning electron microscopy (FESEM) were employed to confirm immobilization of E. aerogenes successfully. The effect of MWCNT-COOH concentrations (0.2, 0.6, and 1.2 mg/mL) on hydrogen production was investigated. The present study showed that immobilized E. aerogenes on 1.2 mg/mL MWCNT-COOH resulted in higher hydrogen yield (2.2 moL/mol glucose), hydrogen production rate (2.72 L/L.h), and glucose degradation efficiency (96.20%) and shorter the lag phase (1 h) compared to the free E. aerogenes. Modified Gompertz and Logistic models were employed to predict the cumulative hydrogen production successfully. •Immobilization of E. aerogenes on MWCNT was used to improve hydrogen production.•Immobilization of E. aerogenes on MWCNT reduces lag phase and increases HY and HPR.•Modified Gompertz and Logistic models were used to predict hydrogen production.