Rhamnolipid induced deagglomeration of anaerobic granular biosolids for energetically feasible ultrasonic homogenization and profitable biohydrogen

• Published in: International journal of hydrogen energy 2020-02, Vol.45 (10), p.5890-5899
• Main Authors: S, Kavitha, Kannah R, Yukesh, M, Gunasekaran, Banu J, Rajesh, Kumar, Gopalakrishnan
• Format: Article
• Language: English
• Subjects: Anaerobic granular biosolids; Biohydrogen; Biosurfactant; Energy analysis; Ultrasonic homogenization
• ISSN: 0360-3199; 1879-3487
• DOI: 10.1016/j.ijhydene.2019.04.063

The present study exposes the effect of deagglomeration using rhamnolipid on anaerobic granular biosolids (AGB) followed by ultrasonic homogenization for effective biohydrogen production. Rhamnolipid was used to remove the extracellular polymeric substance bound over the surface of AGB to increase the rate of biogranular lysis during ultrasonic homogenization. Extracellular polymeric substance (EPS) removal was achieved at an optimum rhamnolipid dosage of 0.04 g Rh/g SS. Ultrasonic homogenization (UH) of AGB demands 27016 kJ/kg TS of specific energy to achieve 16.8% and 13.9% of biogranular lysis and biosolids reduction, respectively. However, rhamnolipid-alkaline pH induced ultrasonic homogenization (RAUH) demand lesser (12607 kJ/kg TS) and achieves greater biogranular lysis (25.4%) and biosolids reduction (20.7%). RAUH significantly saves the net energy. Exponential first order kinetic analysis was done to evaluate and compare the biohydrogen production potential of RAUH with that of UH. The biohydrogen production was found to be 55.1 mL H2/g COD and 36.7 mL H2/g COD for RAUH and UH respectively. A higher positive net energy of 2.62 kWh/kg AGB was achieved by RAUH when compared to UH (−3.49 kWh/kg anaerobic granular biosolids). [Display omitted] •Anaerobic granules are deagglomerated at 0.04 g Rh/g SS of biosurfactant and at pH 9.•Rhamnolipid-alkaline pH induced ultrasonic homogenization demands lesser energy.•Higher biohydrogen of 55.1 mL/g COD was achieved in the proposed study.•A net energy of 2.62 kWh was achieved by this proposed pretreatment.