Protective effect of Coenzyme Q10 against oxidative damage in human lens epithelial cells by novel ocular drug carriers

• Published in: International journal of pharmaceutics 2011-01, Vol.403 (1-2), p.219-229
• Main Authors: Wang, Siling, Zhang, Jing, Jiang, Tongying, Zheng, Li, Wang, Zhanyou, Zhang, Jinghai, Yu, Pan
• Format: Article
• Language: English
• Subjects: adenosine triphosphate; Anti-apoptosis; apoptosis; Biological and medical sciences; cell viability; chitosan; coatings; Coenzyme Q10; confocal laser scanning microscopy; cornea; drug carriers; drugs; epithelial cells; epithelium; fluorescence; General pharmacology; Human lens epithelial cells; humans; hydrogen peroxide; Medical sciences; N-trimethyl chitosan; permeability; Pharmaceutical technology. Pharmaceutical industry; Pharmacology. Drug treatments; protective effect; Transcorneal permeation
• ISSN: 0378-5173; 1873-3476
• DOI: 10.1016/j.ijpharm.2010.10.020

With the enhanced permeation effect of the TMC coating, Coenzyme Q10-loaded TMC-coated liposomes appear to be a promising ophthalmic drug delivery carrier with an efficacy in protecting HLECs against H2O2-induced oxidative damage. The evaluation of N-trimethyl chitosan (TMC)-coated liposomes containing Coenzyme Q10 as potential ophthalmic drug delivery system was carried out. Firstly, transcorneal permeation studies were conducted at 34°C using a side-by-side diffusion apparatus. The transport process of the fluorescent marker, rhodamine B, across the corneal epithelium was visualized with confocal laser scanning microscopy. Secondly, the human lens epithelial cells (HLECs) were cultured without or with Coenzyme Q10 followed by addition of H2O2. The cell viability and apoptosis were evaluated. The permeability coefficient for rhodamine B with TMC-coated liposomes increased more than two times in comparison with the value obtained for solution as control, from (0.42±0.018)×105cms−1 to (1.31±0.030)×105cms−1. Confocal laser scanning microscopy revealed that a TMC coating enhanced the transepithelial transport, dependent on the TMC concentration and contacting time. Coenzyme Q10 elevated the cell viability and reduced the oxidative damage with the decreased percentage of apoptotic cells in a positive concentration-dependent manner. The ATP content of liposome-treated cells was increased about 2-fold compared with that of H2O2-treated cells. Together, our findings demonstrate that with the enhanced permeation effect of the TMC coating, Coenzyme Q10-loaded TMC-coated liposomes appear to be a promising ophthalmic drug delivery carrier with an efficacy in protecting HLECs against H2O2-induced oxidative damage.