Dc-specific aptamer decorated gold nanoparticles: A new attractive insight into the nanocarriers for allergy epicutaneous immunotherapy

• Published in: International journal of pharmaceutics 2020-06, Vol.584, p.119403, Article 119403
• Main Authors: Koushki, Khadijeh, Varasteh, Abdol-Reza, Shahbaz, Sanaz Keshavarz, Sadeghi, Mahvash, Mashayekhi, Kazem, Ayati, Seyed Hasan, Moghadam, Malihe, Sankian, Mojtaba
• Format: Article
• Language: English
• Subjects: Administration, Cutaneous; Allergic rhinitis; Animals; Aptamer; Aptamers, Nucleotide - administration & dosage; Aptamers, Nucleotide - pharmacology; Cytokines - biosynthesis; Dendritic Cells; Desensitization, Immunologic; Epicutaneous immunotherapy; EPIT; Female; Gold - chemistry; Gold nanoparticles; Immunoglobulin E - blood; Inflammation Mediators - metabolism; Metal Nanoparticles - chemistry; Mice; Mice, Inbred BALB C; Rhinitis, Allergic - therapy; Skin penetrating peptides; Targeted immunotherapy
• ISSN: 0378-5173; 1873-3476
• DOI: 10.1016/j.ijpharm.2020.119403

[Display omitted] Recently, the main goal of many allergy epicutaneous immunotherapy (EPIT) studies is to enhance the allergen delivery through the intact skin. Therefore, applying new strategies for tackling this issue are inevitable. For this purpose, ten groups of Che a 2-sensitized BALB/c mice were epicutaneously treated for a 6-week period with the rChe a 2-GNPs-Aptamer, rChe a 2-GNPs-Aptamer + skin-penetrating peptides (SPPs), rChe a 2-GNPs, rChe a 2, GNPs, and PBS. Afterward, the serum IgE and IFN-γ, TGF-β, IL-10, IL-4, IL-17a cytokine production, NALF analysis, and lung/nasal histological examinations were performed. The present study results demonstrate that, EPIT in aptamer treated groups had a significant increase of IFN-γ, TGF-β, and IL-10 concentrations and a significant decrease of IgE, IL-4, and IL-17a concentrations as well as NALF infiltrated immune cell count compared to the non-targeted ones. In addition, SPPs led to more significant improvement of immunoregulatory parameters, especially IL-10 cytokine. Accordingly, the targeted-GNPs with DC-specific aptamers could act as an efficient approach for the improvement of EPIT efficacy compared to the free allergen. Moreover, the application of SPPs might be considered as a useful tool in achieving a successful EPIT with lower doses of allergen at a shorter duration of the treatment.