Enhanced drug retention, sustained release, and anti-cancer potential of curcumin and indole-curcumin analog-loaded polysorbate 80-stabilizied PLGA nanoparticles in colon cancer cell line SW480

• Published in: International journal of pharmaceutics 2020-10, Vol.588, p.119738, Article 119738
• Main Authors: Sufi, Shamim Akhtar, Hoda, Muddasarul, Pajaniradje, Sankar, Mukherjee, Victor, Coumar, Selvaraj Mohane, Rajagopalan, Rukkumani
• Format: Article
• Language: English
• Subjects: Curcumin; Drug delivery; Indole curcumin analog; PLGA nanoparticle; SW480 cells
• ISSN: 0378-5173; 1873-3476
• DOI: 10.1016/j.ijpharm.2020.119738

[Display omitted] The major therapeutic limitation of curcumin and indole-incorporated curcumin analog is its low bioavailability. We hypothesized that nano-encapsulation of indole-incorporated curcumin analog and curcumin as a biodegradable polymeric nanoparticle may enhance its bioavailability with extended drug retention time. Indole-incorporated curcumin analog and curcumin loaded PLGA nanoparticles were synthesized by solvent evaporation technique. Physicochemical characterizations and anti-cancer potential of the nanoparticles were evaluated in human colon cancer cell line SW480. The synthesized NPs had a size range of 50–150 nm diameter. The nano-formulation preserved the drug from degradation in wide ranges of pH environments. The nanoparticles treatment against SW480 cancer cell line triggered nuclear fragmentation, cell cycle blockade, inhibition of apoptosis and metastatic biomarkers. These drug-loaded nanoparticles may be potent nano-formulations against colon cancer because of its ability to tolerate extreme pH environments, thus having potential of oral drug-delivery.