Delivery of acetogenin-enriched Annona muricata Linn leaf extract by folic acid-conjugated and triphenylphosphonium-conjugated poly(glycerol adipate) nanoparticles to enhance toxicity against ovarian cancer cells

[Display omitted] The study demonstrated the fabrication of new poly(glycerol adipate) (PGA) nanoparticles decorated with folic acid (FOL-PGA) and triphenylphosphonium (TPP-PGA) and the potential on the delivery of acetogenin-enriched Annona muricata Linn leaf extract to ovarian cancer cells. FOL-PG...

Full description

Saved in:
Bibliographic Details
Published in:International journal of pharmaceutics 2022-04, Vol.618, p.121636, Article 121636
Main Authors: Damrongrak, Kanokporn, Kloysawat, Kiattiphant, Bunsupa, Somnuk, Sakchasri, Krisada, Wongrakpanich, Amaraporn, Taresco, Vincenzo, Cuzzucoli Crucitti, Valentina, Garnett, Martin C., Suksiriworapong, Jiraphong
Format: Article
Language:English
Subjects:
Adipates
Annona
Annona muricata
Drug Carriers
Folic Acid
Glycerol
Humans
Nanoparticles
Ovarian cancer
Ovarian Neoplasms - drug therapy
Plant Extracts
Poly(glycerol adipate)
Polyethylene Glycols
Polymers
Triphenyl phosphonium
Tumor targeting
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:[Display omitted] The study demonstrated the fabrication of new poly(glycerol adipate) (PGA) nanoparticles decorated with folic acid (FOL-PGA) and triphenylphosphonium (TPP-PGA) and the potential on the delivery of acetogenin-enriched Annona muricata Linn leaf extract to ovarian cancer cells. FOL-PGA and TPP-PGA were successfully synthesized and used to fabricate FOL-decorated nanoparticles (FOL-NPs) and FOL-/TPP- decorated nanoparticles (FOL/TPP-NPs) by blending two polymers at a mass ratio of 1:1. All nanoparticles had small size of around 100 nm, narrow size distribution and high negative surface charge about −30 mV. The stable FOL/TPP-NPs showed highest drug loading of 14.9 ± 1.9% at 1:5 ratio of extract to polymer and reached to 35.8 ± 2.1% at higher ratio. Both nanoparticles released the extract in a biphasic sustained release manner over 5 days. The toxicity of the extract to SKOV3 cells was potentiated by FOL-NPs and FOL/TPP-NPs by 2.0 – 2.6 fold through induction of cell apoptosis. FOL/TPP-NPs showed lower IC50 and higher cellular uptake as compared to FOL-NPs. FOL-NPs exhibited folate receptor-mediated endocytosis. FOL/TPP-NPs provided more advantages than FOL-NPs in terms of stability in physiological fluid, uptake efficiency and targeting ability to mitochondria and showed a promising potential PGA platform for targeted delivery of herbal cytotoxic extracts.
ISSN:0378-5173
1873-3476
DOI:10.1016/j.ijpharm.2022.121636