Extraction, identification and quantitative HPLC analysis of flavonoids from sarang semut (Myrmecodia pendan)

• Published in: Industrial crops and products 2013-01, Vol.41, p.392-396
• Main Authors: Engida, Adam Mekonnen, Kasim, Novy S., Tsigie, Yeshitila Asteraye, Ismadji, Suryadi, Huynh, Lien Huong, Ju, Yi-Hsu
• Format: Article
• Language: English
• Subjects: Antioxidant activity; apigenin; ethanol; Flavonoids; free radical scavengers; free radicals; high performance liquid chromatography; HPLC–UV/vis; Myrmecodia pendan; phenol; Phenolics content; quantitative analysis; quercetin
• ISSN: 0926-6690; 1872-633X
• DOI: 10.1016/j.indcrop.2012.04.043

[Display omitted] ► Water bath extraction was used and three factors (solvent composition, extraction time and solvent volume to sample mass) were considered. ► Using water bath extraction, 13.82% yield was obtained at 80% ethanol, 4h extraction time and 50ml of solvent/g of sample, plant extract showed good DPPH radical scavenging activity. ► Total phenolic and flavonoid contents of sarang semut (Myrmecodia pendens) were determined using predetermined methods and are found in significant amount. ► Five flavonoids were identified and detected in significant amount in the extract using HPLC. The objective of this study was to extract and determine total contents of phenolic and flavonoid compounds as well as to identify and quantify some flavonoids from sarang semut (Myrmecodia pendan). Water bath extraction at 55°C was employed for extracting flavonoids from sarang semut. The effects of parameters such as extraction time, composition of solvent mixture and solvent to sample ratio on extraction were investigated. From (33) factorial design the optimum extracting parameters were determined as follows: extraction time, 4h; ethanol/water composition, 80%; and solvent to sample ratio, 50ml/g. Under these optimal conditions, a yield of 13.82% was obtained. The free radical scavenging activity (antioxidant activity) of the extract was evaluated using DPPH radical and it was found that the IC50 occurred at 96.21±9.03μg/ml of extract. The total phenol and flavonoid contents were determined using designed methods and found to be 330.61±2.13mg GAE/g and 63.28±1.75mg QE/g of dry extract, respectively. The extract obtained under optimum conditions was analyzed by HPLC and five flavonoid compounds were identified and quantified; they are kaempferol (13.767mg/g), luteoline (0.005mg/g), rutine (0.003mg/g), quercetin (0.030mg/g) and apigenin (4.700mg/g) of dry extract.