Antimicrobial and toxicity profiles evaluation of the Chamomile (Matricaria recutita L.) essential oil combination with standard antimicrobial agents

• Published in: Industrial crops and products 2018-09, Vol.120, p.279-285
• Main Authors: Göger, Gamze, Demirci, Betül, Ilgın, Sinem, Demirci, Fatih
• Format: Article
• Language: English
• Subjects: Additive; Aliivibrio fischeri; Bioluminescence assay; Cytotoxic activity; Essential oil; Matricaria recutita L; Synergic
• ISSN: 0926-6690; 1872-633X
• DOI: 10.1016/j.indcrop.2018.04.024

•Chamomile (Matricaria recutita L.) essential oil were combined with ampicillin, cefuroxime, tetracycline, fluconazole and nystatin.•Both of standard and clinical isolates of Escherichia coli, Staphylococcus aureus and Candida albicans were used.•The combinations between essential oil fractions and antimicrobial agents were also demonstrated.•The combinations did not show any cytotoxic effects on healthy cell line at their concentrations which showed antimicrobial effects.•Essential oil and fractions generally have no toxic effect againts Allivibrio fischeri. In this present study, commercial Pharmacopeia (PhEur) grade chamomile essential oil (Matricariae aetheroleum) was combined with different antimicrobial agents including ampicillin sodium, cefuroxime acetyl, tetracycline hydrochloride, fluconazole and nystatin. All combinations were evaluated in vitro against pathogenic standard and clinical resistant Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacterial isolates as well as against Candida albicans for their broad antimicrobial effectiveness. Furthermore, the essential oil was fractioned by column chromatography using n-hexane, diethyl ether, dichloromethane and methanol, respectively. Additionally, all fractions of essential oil were tested in combinations for their minimum inhibitory concentrations (MIC) as well as for their fractional inhibitory concentrations (FIC) against the resistant microbial pathogens. Antimicrobial activities were evaluated by microdilution method and antimicrobial interactions were assayed using the checkerboard method. Cytotoxicity of compounds were evaluated using Cytotox-XTT-1 Parameter Kit in WS1 cells and Aliivibrio fischeri bioluminescence toxicity assay. The analyses proved that α-bisabolol oxide A (47.7%), (E)-β-farnesene (21.5%), α-bisabolol oxide B (6.2%), α-bisabolone oxide A (5.8%), chamazulene (4.1%) and α-bisabolol (2.2%), respectively were the major compounds and in compliance with PhEur. The essential oil combination of fluconazole and nystatin showed “synergic and additive inhibitory effects” against the clinical Candida strain. According to the IC50 values obtained, the inhibitory concentrations of combinations against the clinical Candida strain can be considered to be selective when compared with its effect on WS1 cells. Additionally, the essential oil combination of fluconazole and nystatin showed low toxicity against A. fischeri.