Increased biomass and pigment production from Cassia alata L. callus cultures and their potential as a textile dye

•In an in-vitro system, the hypocotyls explants are the best for callus induction.•MS + 3 μM BAP + 5 μM TDZ + 3 μM IAA is the best for callus biomass production.•Growth curve of the callus is completed in 45–50 days and pigments are produced during stationary phase.•Incorporation of the elicitors Sa...

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Bibliographic Details
Published in:Industrial crops and products 2019-02, Vol.128, p.346-353
Main Authors: Shah, Mansi R., George, Indu Anna
Format: Article
Language:English
Subjects:
Anthraquinones
Callus
Cassia alata
Ethrel
Salicylic acid
Textile dye
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Summary:•In an in-vitro system, the hypocotyls explants are the best for callus induction.•MS + 3 μM BAP + 5 μM TDZ + 3 μM IAA is the best for callus biomass production.•Growth curve of the callus is completed in 45–50 days and pigments are produced during stationary phase.•Incorporation of the elicitors Salicylic acid and Ethrel increases anthraquinone production by 12–13 fold.•Cassia alata callus has a potential to produce pigments which can be used as a textile dye. Cassia alata L. is a medicinally and economically important plant. Various plant parts were analyzed for callus production in this study using different plant growth regulators such as IAA, BAP, TDZ, 2-iP, NAA, Kinetin in the concentration range 1 μM–10 μM and their combinations. The hormone combination which gave the best callus weight was selected for callus growth analysis and pigment production. Elicitation with Salicylic acid and Ethrel increased pigment production 12–13 fold. Callus extract was further assessed as a textile dye. The dye obtained had an excellent fastness to washing and perspiration.
ISSN:0926-6690
1872-633X
DOI:10.1016/j.indcrop.2018.11.018