Anti-Mycobacterium tuberculosis activity of dichloromethane extract of Piper corcovadensis (Miq.) C. DC. roots and isolated compounds

• Published in: Industrial crops and products 2019-05, Vol.131, p.341-347
• Main Authors: Fernandez, Carla M.M., Baldin, Vanessa P., Ieque, Andressa L., Bernuci, Karine Z., Almeida, Rafaela T., Valone, Larissa M., Fonseca, Dyenefer P., Makimori, Regina Y., Andrade, João Paulo P., Pilau, Eduardo J., Romagnolo, Mariza B., Nakamura, Tania U., Cardoso, Rosilene F., Cortez, Diógenes A.G., Gazim, Zilda C., Scodro, Regiane B.L., Dias Filho, Benedito P.
• Format: Article
• Language: English
• Subjects: Isopiperlonguminine; Mycobacterium tuberculosis; Piper corcovandensis; Piperlonguminine; Piperovatine; REMA
• ISSN: 0926-6690; 1872-633X
• DOI: 10.1016/j.indcrop.2019.01.064

[Display omitted] •Crude extract of Piper corcovadensis roots and isolated compounds exhibited anti-Mycobacterium tuberculosis activity.•Crude extract of Piper corcovadensis roots and isolated compounds in combination with rifampicin showed synergism.•Isolated compounds showed selectivity index greater than 20 for Mycobacterium tuberculosis.•Crude extract of Piper corcovadensis roots and isolated compounds presented activity for clinical isolates resistant.•Crude extract of Piper corcovadensis roots and isolated compounds showed promising in vitro results for the development of new drugs. Tuberculosis (TB) is a major public health problem worldwide and the development of drug resistance, as multidrug-resistant (MDR) TB and extensively drug-resistant (XDR) TB, contributed to the worsening of this scenario. Thus, there is an urgent need for the development of new anti-TB drugs. The objective of this research was to characterize the crude extract of the Piper corcovadensis roots (CEPC) by the ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) analysis, in addition to isolate the chemical constituents by column chromatography and evaluate the antibacterial activity against Mycobacterium tuberculosis, as well as their cytotoxicities. Anti-M. tuberculosis activity (H37Rv and clinical isolates) was evaluated using resazurin microtiter assay plate (REMA), classical checkerboard assay and scanning electron microscopy (SEM). The cytotoxicity assay was realized on Vero cells. By UHPLC-MS/MS analysis, four pyrrolidine alkaloids were identified in the chemical composition of CEPC, namely: piperovatine, piperlonguminine, isopiperlonguminine and chingchengenamide A. Piperovatine and the fraction (90% piperlonguminine and 10% isopiperlonguminine) (FRPI) were isolated by column chromatography. For M. tuberculosis H37Rv, CEPC, piperovatine and FRPI showed a minimum inhibitory concentration (MIC) of 15.60; 7.80 and 7.80 μg/mL, and 50% cytotoxic concentration (CC50) of 55; 192 and 215 μg/mL in Vero cells, respectively. The MIC values for clinical isolates ranged between 0.98–3.90 μg/mL for CEPC, 0.48–1.95 μg/mL for piperovatine, and 0.48–3.90 μg/mL for FRPI. Futhermore, CEPC in combination with isoniazid and rifampicin showed synergism, while the piperovatine and FRPI presented synergism in combination with the rifampicin. By analyzing the SEM micrographs, it was possible to conclude that there were alterations in the morphology of the bacteri