Valorization of cellulose in waste paper into value-added cellobionic acid by genetically engineered Pseudomonas taetrolens

• Published in: Industrial crops and products 2022-10, Vol.186, p.115186, Article 115186
• Main Authors: Yoo, Youngchul, Oh, Yu-Ri, Eom, Gyeong Tae
• Format: Article
• Language: English
• Subjects: Cellobionic acid; Cellobiose; Cellulase; Cellulose; Pseudomonas taetrolens; Waste paper
• ISSN: 0926-6690; 1872-633X
• DOI: 10.1016/j.indcrop.2022.115186

To expand the applications of waste paper (WP), we attempted to valorize waste office paper (WOP) into cellobionic acid (CBA) using a two-step biocatalytic method. First, we prepared cellobiose from WOP using commercial cellulases. To increase cellobiose production, we optimized various reaction conditions; the type of cellulases, reaction temperature, the amount of WOP, and the amount of cellulases. Under the optimized reaction conditions, 23 g/L cellobiose was generated from 80 g/L of WOP. CBA was then produced from the prepared cellobiose using recombinant Pseudomonas taetrolens homologously expressing quinoprotein glucose dehydrogenase. From 23 g/L cellobiose derived from WOP, this strain produced 24.1 g/L CBA. We demonstrated for the first time that WOP could be successfully valorized into CBA using cellobiogenic cellulases and a CBA-producing strain. This study sets the groundwork to produce CBA at a low price and is expected to expand the application of CBA in industries. [Display omitted] •CBA was produced from WOP using a two-step biocatalytic method.•Cellobiose was prepared from WOP using commercial cellulase products.•Optimization of reaction conditions for preparation of cellobiose was performed.•Pseudomonas taetrolens showed a high CBA-producing ability from cellobiose.•CBA was produced from the prepared cellobiose using the recombinant P. taetrolens.