Anti-Nephrotoxic effect of green synthesized Actiniopteris radiata silver nanoparticles (AR-AgNPs) against gentamicin induced nephrotoxicity

• Published in: Inorganic chemistry communications 2023-11, Vol.157, p.111244, Article 111244
• Main Authors: Rathish Kumar, S., Anusha, R., Sathish, S., Rugmini, R., Sekhar, K.C., Sulthan Ibrahim, A.
• Format: Article
• Language: English
• Subjects: Actiniopteris radiata– Silver Nanoparticles (AR-AgNPs); Actiniopteris radiate plant; Antioxidants; Ephrotoxicity; Gentamicin; Green Synthesis
• ISSN: 1387-7003; 1879-0259
• DOI: 10.1016/j.inoche.2023.111244

[Display omitted] •The presence of SPR peak in absorption spectrum at 440 nm confirms the formation of AR-AgNPs.•Serum renal marker levels were significantly reverted near control in AR-AgNPs treated mice.•The AR-AgNPs protected kidney tissue against gentamicin induced oxidative damage.•Oral administration of AR-AgNPs to gentamicin induced mice restored the GSH level to normal level. A study on green synthesized silver nanoparticles (AgNPs) using aqueous extract of Actiniopteris radiata and investigated the effect of A. radiata biosynthesized silver nanoparticles (AR-AgNPs) against gentamicin induced nephrotoxicity. The surface plasmon resonance (SPR) peak at 440 nm in UV–visible absorbance spectrum reveals the presence of AgNPs. DLS analysis shows that the distribution of size is normal and the average size of AgNPs converges about 100 nm. SEM revealed the consistent shape, uniform distribution, and closely packed characteristics of NPs whereas EDX confirms the presence of metallic silver with a strong peak at 3 keV. The N–H and O–H stretching vibrations are observed in the FTIR spectrum around 3450 and 3423 cm−1. Gentamicin induced changes in serum parameters resulted in a significant increase in the level of serum urea, creatinine, bilurubin and uric acids. AgNPs even at modest doses (250 mg/kg), significantly reverted the level of serum markers. The gentamicin-induced AR-AgNP in mice with the levels of CAT, GPx, SOD and TBARS ensured the protection against the oxidative damage in mice which clearly implies that enhancing the antioxidant defense mechanism. Nephrotoxicity of gentamicin induced mice were demonstrated by significantly elevated levels of blood urea, serum creatinine, bilirubin, and uric acids. The exposure of AR-Ag nanoparticles leads to a substantial decrease in blood urea and serum creatinine levels and enhanced the antioxidant and anti-nephrotoxic activity.