Genome-wide gene expression analysis of mouse embryonic stem cells exposed to p-dichlorobenzene

• Published in: Journal of bioscience and bioengineering 2016-09, Vol.122 (3), p.329-333
• Main Authors: Tani, Hidenori, Takeshita, Jun-ichi, Aoki, Hiroshi, Abe, Ryosuke, Toyoda, Akinobu, Endo, Yasunori, Miyamoto, Sadaaki, Gamo, Masashi, Torimura, Masaki
• Format: Article
• Language: English
• Subjects: Animals; Chlorobenzenes - administration & dosage; Chlorobenzenes - toxicity; Deep sequencing; Gene Expression Profiling; Gene Expression Regulation - drug effects; Genetic Markers - genetics; Genome - genetics; High-Throughput Nucleotide Sequencing; Mice; Mouse embryonic stem cells; Mouse Embryonic Stem Cells - drug effects; Mouse Embryonic Stem Cells - metabolism; Non-coding RNA; p-Dichlorobenzene; RNA, Long Noncoding - analysis; RNA, Long Noncoding - genetics; RNA, Long Noncoding - isolation & purification; Time Factors; Toxicity Tests; Toxicological assessment
• ISSN: 1389-1723; 1347-4421
• DOI: 10.1016/j.jbiosc.2016.02.007

Because of the limitations of whole animal testing approaches for toxicological assessment, new cell-based assay systems have been widely studied. In this study, we focused on two biological products for toxicological assessment: mouse embryonic stem cells (mESCs) and long noncoding RNAs (lncRNAs). mESCs possess the abilities of self-renewal and differentiation into multiple cell types. LlncRNAs are an important class of pervasive non-protein-coding transcripts involved in the molecular mechanisms associated with responses to chemicals. We exposed mESCs to p-dichlorobenzene (p-DCB) for 1 or 28 days (daily dose), extracted total RNA, and performed deep sequencing analyses. The genome-wide gene expression analysis indicated that mechanisms modulating proteins occurred following acute and chronic exposures, and mechanisms modulating genomic DNA occurred following chronic exposure. Moreover, our results indicate that three novel lncRNAs (Snora41, Gm19947, and Scarna3a) in mESCs respond to p-DCB exposure. We propose that these lncRNAs have the potential to be surrogate indicators of p-DCB responses in mESCs. •We exposed mESCs to p-dichlorobenzene 1 or 28 days (daily dose).•Mechanisms modulating proteins occurred following acute and chronic exposures.•LncRNAs are novel biomarkers of the chemical stress response in mESCs.