Hypoxia regulation of facilitated glucose transporter-1 and glucose transporter-3 in mouse chondrocytes mediated by HIF-1α

Abstract Objectives Chondrocytes reside in a hypoxic environment; they utilize glucose as the main energy source. Facilitated glucose transport represents the first rate-limiting step in glucose metabolism. Results Here we demonstrated that hypoxia induced an elevated expression level of GLUT-1 and...

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Bibliographic Details
Published in:Joint, bone, spine : revue du rhumatisme bone, spine : revue du rhumatisme, 2008-03, Vol.75 (2), p.176-181
Main Authors: Ren, Bu-Fang, Deng, Lian-Fu, Wang, Jun, Zhu, Ya-Ping, Wei, Li, Zhou, Qi
Format: Article
Language:English
Subjects:
Chondrocyte
Glucose transporter
Hypoxia
Hypoxia inducible factor-1α
Internal Medicine
Rheumatology
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Summary:Abstract Objectives Chondrocytes reside in a hypoxic environment; they utilize glucose as the main energy source. Facilitated glucose transport represents the first rate-limiting step in glucose metabolism. Results Here we demonstrated that hypoxia induced an elevated expression level of GLUT-1 and -3 in chondrocytes. Also elevated was hypoxia inducible factor-1α (HIF-1α) protein level. On the contrary, deletion of HIF-1α led to complete loss of the hypoxia-induced gene expression of GLUT-1 and 3. Conclusion These results suggested that chondrocytes had the capacity to detect and respond to low oxygen availability with changes in expression of oxygen-regulated genes, and that hypoxia regulation of facilitated GLUT-1 and -3 was mediated by HIF-1α.
ISSN:1297-319X
DOI:10.1016/j.jbspin.2007.05.012