Formation and analysis of heterocyclic aromatic amine–DNA adducts in vitro and in vivo

The detection and quantification of heterocyclic aromatic amine (HAA)–DNA adducts, critical biomarkers in interspecies extrapolation of toxicity data for human risk assessment, remains a challenging analytical problem. The two main analytical methods currently in use to screen for HAA–DNA adducts ar...

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Bibliographic Details
Published in:Journal of Chromatography B 2004-03, Vol.802 (1), p.155-166
Main Authors: Turesky, Robert J., Vouros, Paul
Format: Article
Language:English
Subjects:
Amines - chemistry
Animals
Chromatography, High Pressure Liquid
DNA
DNA Adducts - analysis
DNA Adducts - chemistry
Heterocyclic aromatic amine–DNA adducts
Humans
Reviews
Spectrometry, Mass, Electrospray Ionization
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Summary:The detection and quantification of heterocyclic aromatic amine (HAA)–DNA adducts, critical biomarkers in interspecies extrapolation of toxicity data for human risk assessment, remains a challenging analytical problem. The two main analytical methods currently in use to screen for HAA–DNA adducts are the 32 P -postlabeling assay and mass spectrometry, using either accelerated mass spectrometry (AMS) or liquid chromatography and electrospray ionization mass spectrometry (LC–ESI–MS). In this review, the principal methods to synthesize and characterize DNA adducts, and the methods applied to measure HAA–DNA adduct in vitro and vivo are discussed.
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2003.10.053