Simple method for the simultaneous isolation and determination of fumonisin B1 and its metabolite aminopentol-1 in swine liver by liquid chromatography-fluorescence detection

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2005-05, Vol.819 (1), p.97-103
• Main Authors: PAGLIUCA, Giampiero, ZIRONI, Elisa, CECCOLINI, Alberto, MATERA, Riccardo, SERRAZANETTI, Gian Paolo, PIVA, Andrea
• Format: Article
• Language: English
• Subjects: Analysis; Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Carboxylic Acids - analysis; Carboxylic Acids - isolation & purification; Chromatography, High Pressure Liquid - methods; Fumonisins - analysis; Fumonisins - isolation & purification; Fundamental and applied biological sciences. Psychology; General pharmacology; Liver - chemistry; Medical sciences; Pharmacology. Drug treatments; Reproducibility of Results; Sensitivity and Specificity; Spectrometry, Fluorescence; Swine
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2005.01.035

An analytical method based on high-performance liquid chromatography (HPLC) combined with fluorescence detection (FL) has been developed for the simultaneous determination of fumonisin B1 (FB1) and its totally hydrolized metabolite aminopentol-1 (AP1) in pig liver. The sample preparation is based on a single solid phase extraction (SPE). o-Phthalaldehyde (OPA) was used for pre-column derivatization before the programmed reversed-phase analysis on phenylhexyl column. The developed method shows good repeatibility for inter- and intra-day precision as well as adequate linearity of calibration curves (r2 was 0.9855 for FB1 and 0.9831 for AP1). Average recoveries from the matrix were 93.6% for FB1 and 95.3% for AP1. The limit of quantification (LOQ) in swine liver was 75 microg/kg for FB1 and 42 microg/kg for AP1.