Solid phase microextraction gas chromatographic analysis of organophosphorus pesticides in biological samples

Headspace-solid phase microextraction (HS-SPME) was studied and optimised for the determination of four common organophosphorus pesticides (OPPs) in biological samples. Various parameters controlling SPME were studied: choice of SPME fiber, type and content of salt added, preheating and extraction t...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2005-08, Vol.822 (1), p.194-200
Main Authors: Tsoukali, Heleni, Theodoridis, Georgios, Raikos, Nikolaos, Grigoratou, Ifigeneia
Format: Article
Language:English
Subjects:
Analysis
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Biological samples
Body Fluids - chemistry
Chemical Fractionation - methods
Chromatography, Gas - methods
Fundamental and applied biological sciences. Psychology
General pharmacology
Humans
Kidney - chemistry
Liver - chemistry
Medical sciences
Organophosphorus pesticides
Organothiophosphorus Compounds - isolation & purification
Pesticides - isolation & purification
Pharmacology. Drug treatments
Sample preparation
SPME
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Summary:Headspace-solid phase microextraction (HS-SPME) was studied and optimised for the determination of four common organophosphorus pesticides (OPPs) in biological samples. Various parameters controlling SPME were studied: choice of SPME fiber, type and content of salt added, preheating and extraction time, desorption time, extraction temperature. Capillary gas chromatographic analysis with nitrogen phosphorus detection (GC–NPD) facilitates sensitive and selective detection of the OPPs: malathion, parathion, methyl parathion and diazinon. Fenitrothion was used as the internal standard. The method was applied to the determination of the pesticides in human biological specimens: whole blood, blood plasma, urine, cerebrospinal fluid, liver and kidney. Limits of detection ranged from 2 to 55 ng/ml depending on pesticide and type of specimen. The developed methodology overcomes limitations and obstacles of conventional methods such as the use of organic solvents, the formation of emulsions and the tedious-cumbersome procedures. The proposed protocol is seen as an attractive alternative to be used in routine toxicological analysis.
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2005.05.035