Determination of tetracyclines residues in honey using high-performance liquid chromatography with potassium permanganate–sodium sulfite–β-cyclodextrin chemiluminescence detection

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2005-09, Vol.824 (1), p.57-64
• Main Authors: Wan, Guo-Hui, Cui, Hua, Zheng, Hai-Song, Zhou, Jian, Liu, Li-Juan, Yu, Xiao-Feng
• Format: Article
• Language: English
• Subjects: Analysis; Analytical, structural and metabolic biochemistry; beta-Cyclodextrins - chemistry; Biological and medical sciences; Chemiluminescence; Chromatography, High Pressure Liquid - instrumentation; Chromatography, High Pressure Liquid - methods; Fundamental and applied biological sciences. Psychology; General pharmacology; Honey; Honey - analysis; HPLC; Luminescent Measurements - instrumentation; Luminescent Measurements - methods; Medical sciences; Methacycline - analysis; Methacycline - chemistry; Oxytetracycline - analysis; Oxytetracycline - chemistry; Pharmacology. Drug treatments; Potassium Permanganate - chemistry; Reproducibility of Results; Residues; Sulfites - chemistry; Tetracycline antibiotics; Tetracyclines - analysis; Tetracyclines - chemistry
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2005.06.039

A novel method was developed for the simultaneous determination of tetracycline antibiotic (TCA) residues such as oxytetracycline (OTC), tetracycline (TC), and metacycline (MTC) by high-performance liquid chromatography (HPLC) coupled with chemiluminescence (CL) detection. The procedure was based on the chemiluminescent enhancement by TCAs of the potassium permanganate–sodium sulfite–β-cyclodextrin system in a phosphoric acid medium. The separation was carried out with an isocratic elution using a mixture of acetonitrile and 0.001 M phosphoric acid. For the three TCAs, the detection limits at a signal-to-noise of 3 ranged from 0.9 to 5.0 ng/ml. The relative standard deviations for the determination of TCAs ranged from 3.1 to 7.4% within a day ( n = 11) and ranged from 2.2 to 8.6% in 3 days ( n = 9), respectively. The method was successfully applied to the determination of TCA residues in honey samples. The possible mechanism of the CL reaction was also discussed.