High-performance liquid chromatography determination of α1-acid glycoprotein in small volumes of plasma from neonates

In order to investigate how the α1-acid glycoprotein (AGP) concentrations of neonates change in response to surgical stress, a simple high-performance liquid chromatography (HPLC)-assay for the measurement of α1-acid glycoprotein levels was developed. A fraction containing α1-acid glycoprotein was i...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2006-02, Vol.831 (1), p.81-84
Main Authors: Stumpe, M., Miller, C., Morton, N.S., Bell, G., Watson, D.G.
Format: Article
Language:English
Subjects:
Alpha1-acid glycoprotein
Anaesthetics
Analysis
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
General pharmacology
HPLC
Medical sciences
Neonates
Pharmacology. Drug treatments
Plasma
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Summary:In order to investigate how the α1-acid glycoprotein (AGP) concentrations of neonates change in response to surgical stress, a simple high-performance liquid chromatography (HPLC)-assay for the measurement of α1-acid glycoprotein levels was developed. A fraction containing α1-acid glycoprotein was isolated from the bulk of plasma protein by addition of 0.6 M perchloric acid and was then analysed directly on a short PLRP-S 4000 Å reversed phase column column. The method was validated by analysis of pooled plasma from healthy adults both in comparison with a calibration curve and by standard additions. The procedure was able to isolate α1-acid glycoprotein rapidly (
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2005.11.039