Simultaneous determination of fluoroquinolones and tetracyclines in chicken muscle using HPLC with fluorescence detection

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2007-02, Vol.846 (1), p.8-13
• Main Authors: Schneider, Marilyn J., Braden, Susan E., Reyes-Herrera, Ixchel, Donoghue, Dan J.
• Format: Article
• Language: English
• Subjects: Analysis; Analytical, structural and metabolic biochemistry; Animals; Anti-Bacterial Agents - analysis; antibiotics; Biological and medical sciences; Chickens; Chromatography, High Pressure Liquid - methods; Fluoroquinolone; Fluoroquinolones - analysis; Fundamental and applied biological sciences. Psychology; General pharmacology; Medical sciences; Muscle, Skeletal - chemistry; Pharmacology. Drug treatments; Reference Standards; Reproducibility of Results; Sensitivity and Specificity; Spectrometry, Fluorescence - methods; tetracycline; Tetracyclines - analysis
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2006.08.005

A multiresidue method has been developed which allows for the simultaneous determination of both fluoroquinolones and tetracyclines in chicken muscle. Samples were extracted with a mix of acetonitrile and 0.1 M citrate, 150 mM MgCl 2, pH 5.0. After centrifugation and evaporation, the extracts could be analyzed by liquid chromatography with fluorescence detection. Good recoveries (63–95%) were obtained from samples fortified with a mix of five fluoroquinolones and three tetracyclines, with satisfactory relative standard deviations. Limits of detection were 0.5 ng/g (danofloxacin), 1 ng/g (oxytetracycline, ciprofloxacin, enrofloxacin), 1.5 ng/g (tetracycline), 2 ng/g (difloxacin) and 5 ng/g (sarafloxacin, chlortetracycline). Enrofloxacin and its metabolite ciprofloxacin, as well as oxytetracycline were determined in enrofloxacin and oxytetracycline incurred chicken muscle using this method.