UV B-irradiation enhances the racemization and isomerizaiton of aspartyl residues and production of Nɛ-carboxymethyl lysine (CML) in keratin of skin

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2011-11, Vol.879 (29), p.3303-3309
• Main Authors: Mori, Yuhei, Aki, Kenzo, Kuge, Katsunori, Tajima, Shingo, Yamanaka, Natsuko, Kaji, Yuichi, Yamamoto, Naoki, Nagai, Ryoji, Yoshii, Hanako, Fujii, Norihiko, Watanabe, Masami, Kinouchi, Tadatoshi, Fujii, Noriko
• Format: Article
• Language: English
• Subjects: advanced glycation end products; antibodies; chromatography; elderly; glucose; humans; immunohistochemistry; irradiation; keratin; lipid peroxidation; lysine; mice; oxidation; proteomics; risk factors; ultraviolet radiation; Western blotting
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2011.05.010

UV-B irradiation is one of the risk factors in age-related diseases. We have reported that biologically uncommon D-β-Asp residues accumulate in proteins from sun-exposed elderly human skin. A previous study also reported that carboxymethyl lysine (CML; one of the advanced glycation end products (AGEs)) which is produced by the oxidation of glucose and peroxidation of lipid, also increases upon UV B irradiation. The formation of D-β-Asp and CML were reported as the alteration of proteins in UV B irradiated skin, independently. In this study, in order to clarify the relationship between the formation of D-β-Asp and CML, immunohistochemical analysis using anti-D-β-Asp containing peptide antibodies and anti-CML antibodies was performed in UV B irradiated mice. Immunohistochemical analyses clearly indicated that an anti-D-β-Asp containing peptide antibody and anti-CML antibody reacted at a common area in UV B irradiated skin. Western blot analyses of the proteins isolated from UV B irradiated skin demonstrated that proteins of 50–70kDa were immunoreactive towards antibodies for both D-β-Asp containing peptide and CML. These proteins were identified by proteomic analysis as members of the keratin families including keratin-1, keratin-6B, keratin-10, and keratin-14.