Development and validation of modified QuEChERS method coupled with LC–MS/MS for simultaneous determination of cymiazole, fipronil, coumaphos, fluvalinate, amitraz, and its metabolite in various types of honey and royal jelly

•Six pesticide residues in four types of honey and royal jelly were determined by LC–MS/MS analysis.•Samples were extracted by a modified EN-QuEChERS method using both C18 sorbent and PSA.•Recovery was good when the honey samples were extracted with 0.2M dibasic sodium phosphate buffer and acetonitr...

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Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2018-01, Vol.1072, p.60-69
Main Authors: Zheng, Weijia, Park, Jin-A., Abd El-Aty, A.M., Kim, Seong-Kwan, Cho, Sang-Hyun, Choi, Jeong-min, Yi, Hee, Cho, Soo-Min, Ramadan, Amer, Jeong, Ji Hoon, Shim, Jae-Han, Shin, Ho-Chul
Format: Article
Language:English
Subjects:
Honey
LC–MS/MS
Pesticides
Quantification
QuEChERS
Residues
Royal jelly
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Summary:•Six pesticide residues in four types of honey and royal jelly were determined by LC–MS/MS analysis.•Samples were extracted by a modified EN-QuEChERS method using both C18 sorbent and PSA.•Recovery was good when the honey samples were extracted with 0.2M dibasic sodium phosphate buffer and acetonitrile.•Three of the market samples tested positive for cymiazole residues. Over the past few decades, honey products have been polluted by different contaminants, such as pesticides, which are widely applied in agriculture. In this work, a modified EN – quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction method was developed for the simultaneous quantification of pesticide residues, including cymiazole, fipronil, coumaphos, fluvalinate, amitraz, and its metabolite 2,4-dimethylaniline (2,4-DMA), in four types of honey (acacia, wild, chestnut, and manuka) and royal jelly. Samples were buffered with 0.2M dibasic sodium phosphate (pH 9), and subsequently, acetonitrile was employed as the extraction solvent. A combination of primary secondary amine (PSA) and C18 sorbents was used for purification prior to liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI+/MS-MS) analysis. The estimated linearity measured at six concentration levels presented good correlation coefficients (R2)≥0.99. The recovery, calculated from three different spiking levels, was 62.06–108.79% in honey and 67.58–106.34% in royal jelly, with an RSD
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2017.11.011